RnoEX0073997 @ rn6
Exon Skipping
Gene
ENSRNOG00000014316 | Rapgef1
Description
Rap guanine nucleotide exchange factor 1 [Source:RGD Symbol;Acc:619793]
Coordinates
chr3:8236382-8241630:+
Coord C1 exon
chr3:8236382-8236518
Coord A exon
chr3:8237000-8237113
Coord C2 exon
chr3:8241452-8241630
Length
114 bp
Sequences
Splice sites
3' ss Seq
CCAGGTTTCCTCTCTTCCAGGAG
3' ss Score
9.2
5' ss Seq
AAGGTCAGT
5' ss Score
8.68
Exon sequences
Seq C1 exon
ACTCTCAGCGCTCTCATCTCTCCTCCTTCACCATGAAGCTGATGGACAAATTCCACTCTCCTAAAATCAAGAGAACACCATCCAAGAAGGGAAAGCCAGCTGAGGTGTCGAAGACTCCTGAGAAGCCTGTGAGCAAA
Seq A exon
GAGGCAAGAGACAGATTTCTACCAGAGGGCTACCCTATCCCCTTGGATCTGGAGCAGCAGGCAGTAGAATTTATGTCCACCAGTGCTGTGGATTCCAGGTCTCAGAGGCAGAAG
Seq C2 exon
AACCTGTGCTGGCTGGAGGAGAAAGAGAAGGAGGTTGTCAGTGCCTTGCGCTACTTTAAGACCATTGTGGACAAAATGGCCATTGATAAGAAGGTTCTGGAGATGCTCCCGGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAGACTGACCCTCGGATCCAGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014316_MULTIEX1-1/2=C1-2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.609 A=0.158 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCACCATGAAGCTGATGGACA
R:
TAAGATGGCCTCCAGCACCTT
Band lengths:
254-368
Functional annotations
There are 1 annotated functions for this event
PMID: 22749401
Inclusion enhances interaction with GRB2, and reduces with CRK. By LUMIER.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]