DreEX0074722 @ danRer10
Exon Skipping
Gene
ENSDARG00000078433 | spc25
Description
SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) [Source:ZFIN;Acc:ZDB-GENE-060810-13]
Coordinates
chr9:49130364-49133933:+
Coord C1 exon
chr9:49130364-49130468
Coord A exon
chr9:49132317-49132415
Coord C2 exon
chr9:49133503-49133933
Length
99 bp
Sequences
Splice sites
3' ss Seq
GTGATTTGATTTATTTGAAGGTG
3' ss Score
6.08
5' ss Seq
ACAGTAAGA
5' ss Score
5.96
Exon sequences
Seq C1 exon
TGATTCAGTCACAAAACAAAGCCAACAAGGACAGGCTGAAGAAGCTGAACAAATGTAAAGATGTCTTTCAGAAGCATCTCAGCCTTGAGATCAGAAAAATTCAAG
Seq A exon
GTGAAAAGCTGCAGTTTGTCTTCAGGAACATCAATGCTAAAGATCCAGACATGGTTCATGCGTTTCTCCTCCAGTTGGATGCAGAGGGCATTTACCACA
Seq C2 exon
TTATCTCCTGTGACCCTCCTCTGGAGAAAATGACACAGCTAGAGAGCAGACTGCAGGAAACCAACAACTTCTCTGCCTTCCTGGCTAACGTCCGGAGAGAGTTTGTGGCGCTTCATACAGGTGCAAAGATGACCTAAATTTCAGAAACGGTCAGAGACCCAGACACCATATTTATAGATATCATCAGCAAATATATCCTGCTTTTTGTTTTTTGAACCACCAGTAATCACTTAGTGTGAAGTATTAAATTGTTTCTGCTTTGTACATTGCTTTATTGATTCATTATTTTTAACAATAAACAACATTTATACAAGTCGTAAAACTTAAAGTGCTTCGTTTTATACTACAGTGACAATATAATGTAAACAATTTGAATGCTGTGTGATCAAAATCATTAATAAAATTAACAACACATTTTTTGTGAAACGTTA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000078433-'7-7,'7-5,8-7
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.194 A=0.000 C2=0.067
Domain overlap (PFAM):
C1:
PF082347=Spindle_Spc25=PU(6.7=13.9)
A:
PF082347=Spindle_Spc25=FE(44.0=100)
C2:
PF082347=Spindle_Spc25=PD(48.0=78.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGCCAACAAGGACAGGCTG
R:
CCAGGAAGGCAGAGAAGTTGT
Band lengths:
172-271
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]