DreEX0081625 @ danRer10
Exon Skipping
Gene
ENSDARG00000028379 | trub2
Description
TruB pseudouridine (psi) synthase family member 2 [Source:ZFIN;Acc:ZDB-GENE-040704-41]
Coordinates
chr6:41026851-41030782:-
Coord C1 exon
chr6:41030648-41030782
Coord A exon
chr6:41029200-41029261
Coord C2 exon
chr6:41026851-41026923
Length
62 bp
Sequences
Splice sites
3' ss Seq
CTCTGATTTCTTTGTCTCAGTAT
3' ss Score
6.99
5' ss Seq
AGGGTAAAG
5' ss Score
5.2
Exon sequences
Seq C1 exon
CGCTGAACTCCAGTCCTCCAGCAGCTCCTCGGCGTGAAGTGCAGTTTCAGCTTCTGTCCAGTGGAGAAAACAGCACATCCAAAGAGCTCATTTTATCTCCATCTATGGTTCCTTCTCTGGCGGATCATCCTTTAG
Seq A exon
TATTAGGTGTTGGGAAAGGAAATAGTGTCCTGGAGAATCTGTGCAAATCACATGTAACCAGG
Seq C2 exon
ACCATATTACCCATGATAAGCTGGAAAGAGTTCTTGCCATGTTACAGGGATCCAATCAGAAAGCCTTGATCAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028379_MULTIEX1-2/5=C1-4
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.152 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0084917=PseudoU_synth_2=FE(24.5=100)
A:
PF0084917=PseudoU_synth_2=FE(10.9=100),PF0150913=TruB_N=FE(13.3=100)
C2:
PF0084917=PseudoU_synth_2=FE(13.0=100),PF0150913=TruB_N=FE(16.0=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCAGTTTCAGCTTCTGTCCA
R:
TGGCAAGAACTCTTTCCAGCT
Band lengths:
135-197
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]