DreEX6024653 @ danRer10
Exon Skipping
Gene
ENSDARG00000087071 | chrna4a
Description
cholinergic receptor, nicotinic, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-130530-903]
Coordinates
chr6:58514189-58514914:+
Coord C1 exon
chr6:58514189-58514252
Coord A exon
chr6:58514379-58514521
Coord C2 exon
chr6:58514870-58514914
Length
143 bp
Sequences
Splice sites
3' ss Seq
GTGTGTGTGTGTGTGTTTAGTGT
3' ss Score
8.78
5' ss Seq
GTGGTATAA
5' ss Score
0.19
Exon sequences
Seq C1 exon
CAGGACGCCATCTTCATCATGTTTTTCTGTCTTCATCTTCTGCTGCTTCTTTATCCGGCTCAAG
Seq A exon
TGTGCTGTGATGAAGTCCAGGCTGAAGAGCGTCTGCTGAACTCTCTGCTCTCAGCATACAGTAAACTGACCCGTCCGGTTCACAGTGTCTCTGATGTGGTTCACGTTCACTTCGGTCTGTCCATCGCTCAGCTCATCGACGTG
Seq C2 exon
GATGAGAAAAATCAAATGATGACCACTAATGTGTGGGTGAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000087071-'0-2,'0-0,1-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(18.8=81.2)
C2:
PF0293118=Neur_chan_LBD=FE(6.8=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGGACGCCATCTTCATCATGT
R:
CTGCTTCACCCACACATTAGT
Band lengths:
109-252
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]