MmuEX6107628 @ mm9
Exon Skipping
Gene
ENSMUSG00000027577 | Chrna4
Description
cholinergic receptor, nicotinic, alpha polypeptide 4 [Source:MGI Symbol;Acc:MGI:87888]
Coordinates
chr2:180768814-180773892:-
Coord C1 exon
chr2:180773697-180773892
Coord A exon
chr2:180772124-180772275
Coord C2 exon
chr2:180768814-180768858
Length
152 bp
Sequences
Splice sites
3' ss Seq
ACTTTCTGCCTCCCCCACAGCTA
3' ss Score
10.14
5' ss Seq
GTGGTAGGT
5' ss Score
7.23
Exon sequences
Seq C1 exon
GGTTTGGGGACGAGCGGCCGCGACACGGGGCATGAAGTTGGGTGCGCGCGGGTCTCGGAGCGGAGGCGCGGTACTGCCGGGAGCCGCCCTCGTCTAGAGCCCGTTCTGTGAGCCATGGAGATCGGGGGCTCCGGGGCGCCGCCGCCGCTGCTGCTCCTGCCGCTCCTGCTGCTCTTAGGGACCGGCCTCTTGCCTG
Seq A exon
CTAGCAGCCACATAGAGACCCGGGCCCATGCGGAGGAGCGGCTCCTGAAGAGACTCTTCTCTGGCTACAACAAGTGGTCTCGGCCAGTAGCCAATATCTCAGATGTGGTCCTTGTCCGCTTTGGCTTGTCGATTGCTCAGCTCATTGATGTG
Seq C2 exon
GATGAGAAAAACCAGATGATGACGACCAACGTGTGGGTGAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000027577-'1-4,'1-3,2-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.036 A=0.025 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(18.8=76.5)
C2:
PF0293118=Neur_chan_LBD=FE(24.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Human
(hg38)
No conservation detected
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACACGGGGCATGAAGTTGG
R:
GCTTCACCCACACGTTGGTC
Band lengths:
218-370
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: