DreEX6040571 @ danRer10
Exon Skipping
Gene
ENSDARG00000040482 | osbpl3b
Description
oxysterol binding protein-like 3b [Source:ZFIN;Acc:ZDB-GENE-090109-1]
Coordinates
chr16:21548156-21559584:+
Coord C1 exon
chr16:21548156-21548263
Coord A exon
chr16:21558649-21558786
Coord C2 exon
chr16:21559491-21559584
Length
138 bp
Sequences
Splice sites
3' ss Seq
TCTGACTTCTCTTAATACAGAAG
3' ss Score
5.03
5' ss Seq
GAGGTGAGC
5' ss Score
8.7
Exon sequences
Seq C1 exon
GCTGTAACGCAGAACGCTGAGCTGAGAGAGCGACTGTGCAAGATTCATGCAGAGTCGCACATCTCTGAGCCCACTGTGCATATAAACCTCAGCCCTGGCCCCCTGCAG
Seq A exon
AAGCAAGACTCCAGTGATTGTTCCCGCCCCCTTGTTCACCAGGCATCCAATGAGAGCAGAGCTTCCATTTCAGAGTCACTGTCAGAGTTCTTTGATGCACAGGAAGTGCTGCTGTCAGCGAGTTCTTCAGAAAATGAG
Seq C2 exon
GCTTCAGAAGATGACTCATATATCAGTGACATCAGTGACAACGTGTCCATGGATAACTTCAGCAACGAGGCAGAGAATGAGAGACTCAATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000040482-'14-16,'14-14,15-16=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.417 A=0.522 C2=0.594
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGAACGCTGAGCTGAGAG
R:
CTGTATTGAGTCTCTCATTCTCTGCC
Band lengths:
194-332
Functional annotations
There are 1 annotated functions for this event
PMID: 17804640
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: classical fluorescence spectroscopy, confocal microscopy, cross linking study, detection by mass spectrometry, green fluorescent protein tag. ELM ID: ELMI001270; ELM sequence: DSLSEFFDAQE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]