DreEX6048560 @ danRer10
Exon Skipping
Gene
ENSDARG00000043593 | rapgef1a
Description
Rap guanine nucleotide exchange factor (GEF) 1a [Source:ZFIN;Acc:ZDB-GENE-081105-26]
Coordinates
chr8:11750771-11751885:-
Coord C1 exon
chr8:11751793-11751885
Coord A exon
chr8:11750932-11751117
Coord C2 exon
chr8:11750771-11750855
Length
186 bp
Sequences
Splice sites
3' ss Seq
GTTTTTGTGTGTGTAAACAGTTT
3' ss Score
7.52
5' ss Seq
CAGGTAAAC
5' ss Score
7.82
Exon sequences
Seq C1 exon
GTACACCAGATTTTCTCTCAGCTCTGACAGCATCAAACAACGAGCTAGTAAAAACACCTTCTTCGTGCTGGTGCGTGTCGTGGATGAGCTTTG
Seq A exon
TTTGGTGGAGCTGACGGAAGACATCCTGAAGCAGCTCATGGAGCTTGTGTTCAATCTCTTGTGTAAAGGGGAGCTTACTTTGGCCCGGGTTCTGCGCAAAAACATCCTGGATAAAGTCCAGCAGAAGAAGTTGCTGCAGTACACTAATTCCCTCAAAACACTGGCTGCACGCGGTGTGGCCGCCAG
Seq C2 exon
GCCTGGCACATTGCATGACTTCCGTAGCCATGAGATCGCAGACCAGCTGACGCTCCTCGATGCAGAGCTCTTTTATAAGATAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000043593-'16-23,'16-22,18-23=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=FE(27.4=100)
A:
PF0061815=RasGEF_N=PD(29.2=52.4)
C2:
PF0061714=RasGEF=PU(12.9=79.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACACCAGATTTTCTCTCAGCTCTG
R:
CTTATAAAAGAGCTCTGCATCGAGGA
Band lengths:
170-356
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]