DreEX6072483 @ danRer10
Exon Skipping
Gene
ENSDARG00000004017 | spag1a
Description
sperm associated antigen 1a [Source:ZFIN;Acc:ZDB-GENE-030131-9443]
Coordinates
chr19:12068411-12076857:+
Coord C1 exon
chr19:12068411-12068510
Coord A exon
chr19:12073525-12073677
Coord C2 exon
chr19:12076703-12076857
Length
153 bp
Sequences
Splice sites
3' ss Seq
CTCATCCGGTTTATTTATAGGGC
3' ss Score
8.36
5' ss Seq
CAGGTACAG
5' ss Score
8.68
Exon sequences
Seq C1 exon
GTATCGACAGCCCAGAGGATCTCTGTGTTCTGTACTCCAACAGAGCTGCGTGCTTCCTCAAAGATGGAAACAGCGCAGACTGCATTCAGGACTGCACCAG
Seq A exon
GGCGCTGGAGCTCCACCCCTTCTCGCTGAAGCCCCTCCTCCGGCGAGCGATGGCCTATGAGTCTCTGGAGCGCTACAGGAAAGCCTATGTGGATTACAAAACAGTGCTGCAGATTGACATCAGTGTGCAGGCCGCTCACGACAGTGTACACAG
Seq C2 exon
AATCACTAAAATGCTGATTGAGCAGGACGGTCCTGACTGGCGAGAGAAGCTTCCAGAGATTCCAGCGGTTCCTCTTTCTGCTCAGCAGCACCGCAAAGAAGAGCCCAGCGCAGAGCTCCTGCAGGCCCGAGCAGAAAGAGCAGAACAGGAGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004017-'1-2,'1-1,2-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.419
Domain overlap (PFAM):
C1:
PF134141=TPR_11=FE(44.0=100)
A:
PF134141=TPR_11=PD(8.0=11.5),PF0051523=TPR_1=WD(100=59.6)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCGACAGCCCAGAGGATCTC
R:
GTTCTGCTCTTTCTGCTCGGG
Band lengths:
244-397
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]