GgaEX1054858 @ galGal4
Exon Skipping
Gene
ENSGALG00000016037 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr2:128193925-128200218:+
Coord C1 exon
chr2:128193925-128194024
Coord A exon
chr2:128197023-128197175
Coord C2 exon
chr2:128200043-128200218
Length
153 bp
Sequences
Splice sites
3' ss Seq
CTCTTCTGGTTTTTTTCCAGAGC
3' ss Score
9.97
5' ss Seq
TAGGTAAAT
5' ss Score
6.62
Exon sequences
Seq C1 exon
GAGAACGAAGTCCAGATGATTTAAGTATCTTGTACTCAAACAGAGCAGCGTGTTACTTAAAAGAAGGCAACTGCAGTGACTGTGTTCAAGATTGTAACAG
Seq A exon
AGCTCTGGAGCTTCAGCCATTTTCCCTTAAGCCTCTTCTACGACGAGCAATGGCATATGAGTCTATGGAGCGGTATCGACAGGCATATGTTGATTACAAAACAGTCCTACAAATAGACAGCAGCATACAAGTAGCAAATGACAGTGTTAATAG
Seq C2 exon
AATAACGAAGACTTTAATTGATCAAGATGGTTCTAGTTGGAGGGAAAAACTGCCACCGATTCCAGTTGTCCCTATTGCTGCTCAGCTAAACAGGTGGGATGGAGGAAACTTTACTTCAGAGGTTAAGCCAAAAAGTCCCACAGATATCAACAAAGAAGAACAGTTGCAGATGAATC
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016037_MULTIEX1-1/5=C1-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.350
Domain overlap (PFAM):
C1:
PF134141=TPR_11=FE(43.4=100)
A:
PF134141=TPR_11=PD(7.9=11.5),PF0051523=TPR_1=WD(100=61.5)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCAAACAGAGCAGCGTGTTAC
R:
GATTCATCTGCAACTGTTCTTCT
Band lengths:
242-395
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]