DreEX6072853 @ danRer10
Exon Skipping
Gene
ENSDARG00000062640 | kcnh8
Description
potassium voltage-gated channel, subfamily H (eag-related), member 8 [Source:ZFIN;Acc:ZDB-GENE-060503-204]
Coordinates
chr19:21034602-21041921:+
Coord C1 exon
chr19:21034602-21034640
Coord A exon
chr19:21041439-21041668
Coord C2 exon
chr19:21041806-21041921
Length
230 bp
Sequences
Splice sites
3' ss Seq
TCTCCTCTGTCCAATCACAGATG
3' ss Score
9.52
5' ss Seq
AAGGTGTGC
5' ss Score
6.3
Exon sequences
Seq C1 exon
GTCATATCCAGGATGTCCAGTAAGTCCATGGACACACAG
Seq A exon
ATGGAGGGGAGGGGAAACGGAGATGCGATCCAGTGCTATCCACCAATAGCTGAGCATCACAATGAAGAAGATGACGATGACGACGACGATGAAGATGATCAGATACAGTACCTCAGCGGTGGAGGCAAACAGAAAACACACATGCAGAAAAATGATCACCAAACCAACAAAAAGCTCCACATTCAGCACACCACTGTCACGAAACCTGACCTCGGAAATCTCAGCCCAAG
Seq C2 exon
GGTCGTGGACGGCACGGAGGACAGTGATGGCACAGAAAACTCCCACACGTTCATATTTTCCTCTGCTCATCAGCACAGCGTCAGTGGTGCGACCAACACCACTGCCACCACAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062640-'17-12,'17-11,18-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TATCCAGGATGTCCAGTAAGTCCA
R:
CTGTGGTGGCAGTGGTGTTG
Band lengths:
148-378
Functional annotations
There are 1 annotated functions for this event
PMID: 27453045
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation. ELM ID: ELMI003176; ELM sequence: LPSIVE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]