DreEX6082841 @ danRer10
Exon Skipping
Gene
ENSDARG00000015971 | rapgef1b
Description
Rap guanine nucleotide exchange factor (GEF) 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1001]
Coordinates
chr21:4023520-4036408:+
Coord C1 exon
chr21:4023520-4023746
Coord A exon
chr21:4036128-4036214
Coord C2 exon
chr21:4036323-4036408
Length
87 bp
Sequences
Splice sites
3' ss Seq
CCCTGTTGTTTTTCCTGTAGAGC
3' ss Score
10.05
5' ss Seq
ACTGTAAGA
5' ss Score
4.8
Exon sequences
Seq C1 exon
TCCTGCAATACATGCAGTTTGTGGAGGATTACTCTGAGCCGCAGCCGTCCGTCTTCTACCAGCTGCCTCAGAGCGAGAGTATTTACGAGCAGCGCAGGAACAAGCGCTTTCAGGAGGTTTACGGCCTCAACGACTCCTACAGCAGCTCCGACTCCATTCCTGCTTCAGCTGCAGATCCGCACGAACGGGTCCCACCACCAGCGCTGCCTCCAAAACTCAGACAACTG
Seq A exon
AGCGAAAGCATTGATGAAAGTGGTGAAGGAGAATATGTAAACCTGTACTCGTCCACACAGGCCAACGGAGAAAACACACACCGCACT
Seq C2 exon
GAATCTTTATCATGTGACGATGTGCTTCAAGACCACTCCCCTCACATTCCCTCGTCCAATAGCAAGGAGGCTCTGACTAAGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015971-'16-20,'16-19,20-20=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.801 A=0.951 C2=0.981
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCGCTTTCAGGAGGTTTACG
R:
GGAATGTGAGGGGAGTGGTCT
Band lengths:
175-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]