DreEX6083166 @ danRer10
Exon Skipping
Gene
ENSDARG00000037963 | nek6
Description
NIMA-related kinase 6 [Source:ZFIN;Acc:ZDB-GENE-040724-133]
Coordinates
chr21:8267631-8270440:-
Coord C1 exon
chr21:8270333-8270440
Coord A exon
chr21:8268511-8268605
Coord C2 exon
chr21:8267631-8267744
Length
95 bp
Sequences
Splice sites
3' ss Seq
TTTTTGTTTGTTTTTCACAGTTG
3' ss Score
9.79
5' ss Seq
GAGGTATAT
5' ss Score
7.3
Exon sequences
Seq C1 exon
ATATAAAACCAGCCAACGTCTTCATCACAGCTAGCGGAGAGGTCAAACTCGGAGACCTGGGACTGGGGCGATTCTTCAGCTCCAAAACCACTGCTGCACACTCTCTAG
Seq A exon
TTGGAACTCCATATTACATGTCTCCAGAGAGGATTCATGAAAACGGCTACAACTTCAAATCAGACATCTGGTCTCTGGGCTGCTTACTGTATGAG
Seq C2 exon
ATGGCAGCTCTGCACAGCCCCTTTTACAGTGACAAGATGAACCTGCTCTCGCTGTGCCATAAGATTGAACAGTGTGAATACCCACCTCTGCCAACAGAGCACTACTCACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000037963-'8-9,'8-8,9-9=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(13.8=100)
A:
PF0006920=Pkinase=FE(11.9=100)
C2:
PF0006920=Pkinase=FE(14.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTAGCGGAGAGGTCAAACT
R:
TGGCAGAGGTGGGTATTCACA
Band lengths:
173-268
Functional annotations
There are 1 annotated functions for this event
PMID: 16476580
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: coimmunoprecipitation, glutathione s tranferase tag, pull down. ELM ID: ELMI002027; ELM sequence: YYMSPE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]