Special

MmuEX6100956 @ mm10

Exon Skipping

Gene
ENSMUSG00000026749 | Nek6
Description
NIMA (never in mitosis gene a)-related expressed kinase 6 [Source:MGI Symbol;Acc:MGI:1891638]
Coordinates
chr2:38569628-38582496:+
Coord C1 exon
chr2:38569628-38569735
Coord A exon
chr2:38577239-38577333
Coord C2 exon
chr2:38582383-38582496
Length
95 bp
Sequences
Splice sites
3' ss Seq
CACCCCTTGCTGTGTTGCAGTGG
3' ss Score
7.57
5' ss Seq
GAGGTAAGT
5' ss Score
11.08
Exon sequences
Seq C1 exon
ACATCAAGCCCGCCAACGTGTTCATCACAGCTACGGGCATTGTGAAGCTTGGTGACCTCGGCCTGGGCCGCTTCTTCAGCTCGGAGACCACTGCGGCCCACTCACTAG
Seq A exon
TGGGAACACCATACTACATGTCACCCGAGAGAATCCATGAGAATGGCTACAACTTCAAGTCAGACATCTGGTCCCTAGGCTGCCTGCTGTATGAG
Seq C2 exon
ATGGCAGCTCTCCAGAGCCCCTTCTATGGAGATAAGATGAATCTCTTCTCCCTCTGCCAAAAGATTGAGCAGTGTGACTACCCGCCTCTCCCGGGAGAGCACTACTCTGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026749-'24-27,'24-22,29-27=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0006920=Pkinase=FE(13.8=100)

							
A:
PF0006920=Pkinase=FE(11.9=100)

							
C2:
PF0006920=Pkinase=FE(14.2=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000049723





     
                   









    
Main Skipping Isoform:
ENSMUST00000054234fB13526





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000108516, ENSMUSP00000108523
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm9




Conservation


Human
(hg38)
chr9:124339571-124339665 
HIGH PSI
HsaEX6062953
(NEK6)
Human
(hg19)
chr9:127101850-127101944 
HIGH PSI
HsaEX6062953
(NEK6)
Rat
(rn6)
chr3:22879698-22879792 
HIGH PSI
RnoEX6016665
(Nek6)
Cow
(bosTau6)
chr11:95388305-95388399 
HIGH PSI
BtaEX6046072
(NEK6)
Chicken
(galGal4)
chr17:9365449-9365543 
HIGH PSI
GgaEX0001212
(NEK6)
Chicken
(galGal3)
chr17:10082225-10082319 
HIGH PSI
GgaEX0001212
(Q5F3L5_CHICK)
Zebrafish
(danRer10)
chr21:8268511-8268605 
HIGH PSI
DreEX6083166
(nek6)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ATCACAGCTACGGGCATTGTG

				
R: 
CGGGTAGTCACACTGCTCAAT

				
Band lengths: 
169-264

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 1 annotated functions for this event 


PMID: 16476580
HsaEX6062953
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: coimmunoprecipitation, glutathione s tranferase tag, pull down. ELM ID: ELMI002027; ELM sequence: YYMSPE; Overlap: FULL


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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