DreEX6102121 @ danRer10
Exon Skipping
Gene
ENSDARG00000023886 | cacna2d4b
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4b [Source:ZFIN;Acc:ZDB-GENE-100422-18]
Coordinates
chr25:18951418-18953630:-
Coord C1 exon
chr25:18953538-18953630
Coord A exon
chr25:18951578-18951708
Coord C2 exon
chr25:18951418-18951486
Length
131 bp
Sequences
Splice sites
3' ss Seq
CTGAAACTTTGGCCTCACAGAAA
3' ss Score
6.21
5' ss Seq
AAGGTGCAT
5' ss Score
4.62
Exon sequences
Seq C1 exon
TGTGGATGAAGGCATGGAGTCGGCTTTCCTTGGGACACGCTCTGGAGTCTTGCGTGTCATCAGATATACAGGAATTGAAAAACGATTGGGAAA
Seq A exon
AAAGTTCTTGACCCGTGTGGATAAAGAAAACCTCTTCACAGTGGACCACTTCCCCATTTGGTATCGGCTGGCAGCGGAGAATACACCTGGTCAATTCCTCTTTTACGTCCCATATGATGACATAAACAAAG
Seq C2 exon
GCAAGAACACTTTTGTTGCAGTAACATCAGTGACAGTGACTGAAAGGAAAAGAACAGCCATTGCTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000023886-'27-27,'27-26,28-27=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.031
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGGATGAAGGCATGGAGTCG
R:
GCAATGGCTGTTCTTTTCCTTTCA
Band lengths:
156-287
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]