DreEX6102135 @ danRer10
Exon Skipping
Gene
ENSDARG00000045803 | mfge8b
Description
milk fat globule-EGF factor 8 protein b [Source:ZFIN;Acc:ZDB-GENE-070209-137]
Coordinates
chr25:19083360-19094242:+
Coord C1 exon
chr25:19083360-19083544
Coord A exon
chr25:19086074-19086229
Coord C2 exon
chr25:19090311-19094242
Length
156 bp
Sequences
Splice sites
3' ss Seq
TTTTGATCTGTGTGTTTTAGGTG
3' ss Score
11.66
5' ss Seq
AAGGTTGGG
5' ss Score
4.94
Exon sequences
Seq C1 exon
GTTGCTCCGAGCCGCTCGGCGTCAAGTCTCGGCTCATCAGTGACCGGCAGATGACATCGTCCAGCGCTTTCCGCACCTGGGGCATTGAGGCCTTCACCTGGCACCCGCATTACGCCCGTCTGGACAAACAGGGCAAGACCAACGCCTGGACGGCAGCTACAAACAACCGCTCCGAGTGGCTGCAG
Seq A exon
GTGGATCTGCTCAGACCAAAGCGCATTACAGGAATCATCACACAAGGAGCCAAAGACTTCGGCAATGTGCAGTTTGTATCTGCCTATAAAGTGGCTCACAGTGACGACGGCCAACACTGGACGATACTCAAAGAAGACAAGACCAAAACAGATAAG
Seq C2 exon
ATCTTCCCTGGCAACAGTGACAACAACGTGCACAAAAAGAATGTGTTTGACCCGCCCTTCTACGCGCGATTCGTCCGCGTGCTGCCGTGGGCGTGGCATGAGCGCATCACTCTGCGAATGGAGCTGCTGGGCTGCGACGAGTAAACCCCGCCCACTTCTCTCTCAACCTCTCCTCTCTATTTCTCCTTCTCCCTCTTTATACTTCCCATCATTCCTTCTTTTCTCAAACACACCTGCACTGCCTCACTCTCAGCAACAGAGGTCACCATCGCCACACGAGCCAAACCACCACCAACAACACACTAAGCTCAAACCCACAGCGTCACCCTTCCTAAAACAATTCAATACTTATATACAGTGTTCTCCGCTAATATTGGCAGCTTTGGTAAGTAAAACTATATTTTAAAATATATGTTTATATATATTGGCATTAGTGAATATGAGCAAGGAATACTGGGGTGAAAATGAGCGAGTTTGTCTTGATATTACCTCATATTTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000045803-'8-10,'8-9,9-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=0.038 C2=0.128
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=PU(32.1=72.6)
A:
PF0075420=F5_F8_type_C=FE(36.4=100)
C2:
PF0075420=F5_F8_type_C=PD(29.3=85.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTACGCCCGTCTGGACAAAC
R:
AGGTTGAGAGAGAAGTGGGCG
Band lengths:
246-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]