RnoEX6003376 @ rn6
Exon Skipping
Gene
ENSRNOG00000017510 | Mfge8
Description
milk fat globule-EGF factor 8 protein [Source:RGD Symbol;Acc:3083]
Coordinates
chr1:140846182-140848642:-
Coord C1 exon
chr1:140848458-140848642
Coord A exon
chr1:140846356-140846505
Coord C2 exon
chr1:140846182-140846253
Length
150 bp
Sequences
Splice sites
3' ss Seq
TTCTTTTTTTTTTCCTGTAGGTT
3' ss Score
12.69
5' ss Seq
AAGGTGGGT
5' ss Score
8.23
Exon sequences
Seq C1 exon
GATGCTCTGAGCCCCTGGGCCTGAAGAATAACACGATTCCTGACAGCCAAATAACAGCCTCCAGCAGCTACAAGACGTGGAACCTGCGTGCCTTTGGCTGGTACCCCCACTTGGGGCGGCTGGACAATCAGGGCAAGATCAATGCCTGGACAGCTCAGAGCAACAGTGCCAAGGAATGGCTGCAG
Seq A exon
GTTGACCTGGGCACTCAGAAAAAAGTGACAGGAATTATCACCCAGGGGGCCCGTGACTTTGGCCACATCCAGTATGTGGCATCCTATAAGGTAGCCCACAGTGATGATGGTGTGCAGTGGACCGTATATGAGGAACAAGGAACCAGCAAG
Seq C2 exon
GTCTTCCAGGGCAACTTGGACAACAACTCCCACAAGAAGAACATCTTTGAGAAACCTTTCATGGCTCGCTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000017510-'25-12,'25-11,26-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=0.000 C2=0.068
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=PU(32.6=72.6)
A:
PF0075420=F5_F8_type_C=FE(35.5=100)
C2:
PF0075420=F5_F8_type_C=FE(19.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCCTGGGCCTGAAGAATAAC
R:
AGCGAGCCATGAAAGGTTTCT
Band lengths:
244-394
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]