DreINT0012749 @ danRer10
Intron Retention
Gene
ENSDARG00000003795 | IDH2
Description
isocitrate dehydrogenase 2 (NADP+), mitochondrial [Source:HGNC Symbol;Acc:HGNC:5383]
Coordinates
chr18:26116799-26117282:-
Coord C1 exon
chr18:26117146-26117282
Coord A exon
chr18:26116951-26117145
Coord C2 exon
chr18:26116799-26116950
Length
195 bp
Sequences
Splice sites
5' ss Seq
GAAGTGAGG
5' ss Score
3.71
3' ss Seq
ATCTTTTTTTGCATGGGCAGAAA
3' ss Score
5.9
Exon sequences
Seq C1 exon
TCTATCACCGGCTTCGCTCACAGCTGCTTCCAGTACGCCATCCAGAAGAAATGGCCTCTCTACATGAGCACCAAGAACACCATCCTGAAGGCTTATGACGGCAGATTCAAGGACATCTTCCAGGATATCTTTGAGAA
Seq A exon
GTGAGGAGGATTCGATTCAGTCTTTGGCACGCTTTATTTACGCACTTCCATTGTGTTTTGTATGAACGTTTGTTGCTGTGTAATTATGAGATTAGCGCTCTGACATGCTGTCACAGTGTTGACATGTGAAACTACACTTTTCTTGCAAAGTAATTTCAATGCAAAATATTGAAGCATCTTTTTTTGCATGGGCAG
Seq C2 exon
AAACTACAAGCCAGAGTTTGACAAGCTGAAGATCTGGTATGAACACAGACTGATTGATGACATGGTGGCTCAGGTGCTGAAGTCATCCGGTGCCTTTGTGTGGGCCTGCAAGAACTATGATGGAGATGTGCAGTCGGACATTCTTGCTCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003795:ENSDART00000163226:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=FE(19.0=100)
A:
NA
C2:
PF0018015=Iso_dh=FE(21.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGCCATCCAGAAGAAATGGC
R:
GAGCAAGAATGTCCGACTGCA
Band lengths:
252-447
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]