MmuINT0080188 @ mm10
Intron Retention
Gene
ENSMUSG00000030541 | Idh2
Description
isocitrate dehydrogenase 2 (NADP+), mitochondrial [Source:MGI Symbol;Acc:MGI:96414]
Coordinates
chr7:80097804-80098272:-
Coord C1 exon
chr7:80098136-80098272
Coord A exon
chr7:80097956-80098135
Coord C2 exon
chr7:80097804-80097955
Length
180 bp
Sequences
Splice sites
5' ss Seq
CAAGTAACA
5' ss Score
2.32
3' ss Seq
ACAAGCCCTTTTGCCCCCAGGCA
3' ss Score
8.75
Exon sequences
Seq C1 exon
TCCATTTCGGGCTTCGCGCACAGCTGCTTCCAGTACTCTATCCAGAAGAAATGGCCGCTCTACTTGAGCACCAAGAACACCATTCTGAAGGCCTATGACGGGCGTTTCAAGGACATCTTCCAGGAGATCTTTGACAA
Seq A exon
GTAACAACCTCTGATGCTTCCTGGGGGCACCACAGCCCCCCGGCTTCCTTGCGCCATTCCTACTTCACCTGGGGAGGTTGCTTTCCTAGTGTGCGCTTGGTTCAGTTGTGAGTTTCAGGGAGGGGTCTTTAACCTGCCAACCCCCTGCCCTCTCCCTGAGACAAGCCCTTTTGCCCCCAG
Seq C2 exon
GCACTATAAGACTGACTTCGACAAGAATAAGATCTGGTATGAACATCGGCTCATCGACGACATGGTGGCCCAGGTGCTCAAGTCTTCCGGTGGCTTTGTGTGGGCTTGCAAAAACTATGATGGAGACGTGCAGTCTGACATCCTGGCTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030541:ENSMUST00000107384:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.008 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=PD(10.7=31.9)
A:
NA
C2:
PF0018015=Iso_dh=FE(12.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCAGAAGAAATGGCCGCTC
R:
CAGGATGTCAGACTGCACGTC
Band lengths:
243-423
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types