RnoINT0072861 @ rn6
Intron Retention
Gene
ENSRNOG00000013949 | Idh2
Description
isocitrate dehydrogenase (NADP(+)) 2, mitochondrial [Source:RGD Symbol;Acc:1597139]
Coordinates
chr1:141877382-141877843:-
Coord C1 exon
chr1:141877707-141877843
Coord A exon
chr1:141877534-141877706
Coord C2 exon
chr1:141877382-141877533
Length
173 bp
Sequences
Splice sites
5' ss Seq
CAAGTAACA
5' ss Score
2.32
3' ss Seq
ACAAGTCCTTTTGCCCCCAGGCA
3' ss Score
8.08
Exon sequences
Seq C1 exon
TCCATTTCGGGCTTCGCGCACAGCTGCTTCCAGTACTCTATCCAGAAGAAATGGCCGCTCTACTTGAGCACCAAGAACACCATTATGAAGGCCTATGACGGGCGTTTCAAGGACATCTTCCAGGAGATCTTTGACAA
Seq A exon
GTAACAGCCTTTGATGCTTCCTGAGGGCACCGCAGTACCCCAGCTCCCTTGCGCCATCCCTACTTGGGGAGGTTGCTTTTACAGTGTGCTTGGTTTAGTTTTGAGGTTCAGGGAGGGGCCTTTAACCTGCCAGATTCCTGTGCTCTCCCCGAGACAAGTCCTTTTGCCCCCAG
Seq C2 exon
GCACTATAAGACTGACTTTGACAAGAATAAGATCTGGTACGAGCATCGGCTCATTGACGACATGGTGGCTCAGGTGCTCAAGTCTTCCGGTGGCTTTGTGTGGGCCTGCAAGAACTATGACGGAGATGTACAGTCTGACATCCTGGCTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013949:ENSRNOT00000019059:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=FE(11.3=100)
A:
NA
C2:
PF0018015=Iso_dh=FE(12.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCAGAAGAAATGGCCGCTC
R:
TGAGCCAGGATGTCAGACTGT
Band lengths:
248-421
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]