DreINT0038766 @ danRer10
Intron Retention
Gene
ENSDARG00000075883 | bub3
Description
BUB3 mitotic checkpoint protein [Source:ZFIN;Acc:ZDB-GENE-041010-210]
Coordinates
chr12:49196192-49197913:-
Coord C1 exon
chr12:49197588-49197913
Coord A exon
chr12:49196387-49197587
Coord C2 exon
chr12:49196192-49196386
Length
1201 bp
Sequences
Splice sites
5' ss Seq
ACGGTGAGA
5' ss Score
8.94
3' ss Seq
GTGTGTGTGTGTGCGTGCAGATG
3' ss Score
9.51
Exon sequences
Seq C1 exon
GAGGGGAGTCGCGGCTGCTCCTCTCCGCCACTCGGTCTCTCCGGTTAACGGAAATATAAACTCACTAATCCTGAAACAGATTTAATCCATACCTGAGCTGTCGATCAAACGGCTGATGCGCCGCCGGAAACACGTCACTTCTCGCGGTGATTTGTTGTTTCGTGTGCTCGTTCGCGCCAGGAAGTTGATGCGCGTAACCAGTCAGCAGTAGAGACCGTTGGACGGAGTTTTGAAACGGGCCAATGAGAGCAGAGCTCTGCTGTGAACGCAGCCAATCAAATTGAGCCCAGATGAGCGGCAGAGCAGCAGCAGAGAACAGCGGCACG
Seq A exon
GTGAGAGCTAACGCGCATTATATCAGTATACTGTTATTAATATTAATACTAGACCAATCGCGAGTGTCTGAGAGAGGTTTACTCGCCTGCTTCAGCTCTGAGCAGCGCGGGAGAGATGATCAGAGCAGCGAGAGTCAGGATCCACAGCTCCCGGGGCTACAGCGAGTCCAGTTATGGGGAGCGGATGCTAACTGAGGGTGTCTGGTTCCTTCTCTCCTGTGTTCATTCTAGGAGCAGCTGGATAATGGTCTAGCTGGAGTAATACACAAGTGAGGGTCTGTTATGATAAGAGCTCTACTGAGGGCCTAATTAACTGTTAGTAGACTAGTGAAGGTCGGTAATGAGAATAGCACTAGTGTAATCTGACTAGTGCTCTAGCAAGGGTTTGTGTTAAGAATAGTGCACTAGTGAGGGTCTGTGTTATAAGAGCACTAGTAAGGGTCTGTGATGAAAATAGCGCTGGTGATTGTCTGATTAGTGCTCTAGCAAGGGTCTGTAATATGATAAGAGCACTACTGAGGGTTTAATTGGTAGACTAGTGAGCGTCTGTCTTATGATAGAAGAACACTACTGAGGGTCTGTGACAAGAATAACGCTAGTGATGGTCTGAATAGTGCACTAGTGAGGGTCTGTTGAGAGTAGGGCTGCTCAATCTATCGTTTCAGCATGGATATGGCAGTGTGCGTATCCGCTATAGCAGGGGTCACCAATCCTGGTCCTGGAGGTCCGGTGTCCCTGCAGGGTTTAGGATTATTAGGAAAACAGGATTATTTCACGCTGACAGATCATCTCCTTAAAACAAGCAGCAAACTCCTCATTTCACTGATTTCCTCTGACGGTGAACACTCCACAATCATCTTGATATTTGGACTAGAAACGAGACAAAACGAAGTCAGAAAAGCTTTTCTCTGCCTTAATACTGAAAGACTCCAGAACACCGCCAGACTCGCAGTATCTCAGCTGTGTTCACACTGCAGAAGAAAACAAGGCAGAATCACATTATTGCAGGACCCTGCAGCTCTATACACAGTAGTGATGATGATGATGATGATGGTGATGGAGCTCTACTGAGGGTCTGGAGAGTAGTGTAGTGGTCAGCGAGTGCCCTTGAGTGTCTGTAGTAATGATTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGCAG
Seq C2 exon
ATGACCGGAGCGAATGAGTTTAAGCTGGCCCAGGGCCCGGAGGACAGCGTCTCGGCAGTGAAGTTCAGCCCGTCCTCCTCTCAGTTCCTGCTGGTGTCCTCTTGGGACGGCTCCGTGCGGCTCTACGACGCCAGCGCCAACAGCATGAGGATGAAGTACCAACACCTGGCGCCCGTGCTGGACTGCGCTTTCTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075883:ENSDART00000153244:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.958 A=NA C2=0.152
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0040027=WD40=WD(100=55.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGTCACTTCTCGCGGTGATTT
R:
TTCATCCTCATGCTGTTGGCG
Band lengths:
349-1550
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]