GgaINT0042399 @ galGal4
Intron Retention
Gene
ENSGALG00000009681 | BUB3
Description
mitotic checkpoint protein BUB3 [Source:RefSeq peptide;Acc:NP_001006506]
Coordinates
chr6:31024414-31026513:+
Coord C1 exon
chr6:31024414-31024522
Coord A exon
chr6:31024523-31026318
Coord C2 exon
chr6:31026319-31026513
Length
1796 bp
Sequences
Splice sites
5' ss Seq
ACGGTACTG
5' ss Score
5.88
3' ss Seq
CATTTGTGCTCTGTTGTCAGATG
3' ss Score
7.74
Exon sequences
Seq C1 exon
ACAGGACTCTCCGGGACCGGGCTGCCTTCCTCCCCCGCGCTGCTGCGTGAAGAACGTTTCTCCTTGGTGTGGCCTCGAGAGAGGGAAGAAGCCGTGTACTATGAAAACG
Seq A exon
GTACTGCTGGGCTCGGGGCCGGGCCTCCGTGCTCTCAGTGAGGCGGCCGCTGTGTGCTGTGTGCCGGGGTCCTGGGGGCTGCAAAAGCGCTGGTTTCGTGTCTGTGTTGTACAGTGAGCTCTCGGGGGTGGTAGAATTGCAGAGGCACCCGGTGCTGAAGGTGGTGTCATGGTTCTCCCTTTGCTCGAATGTCTTCTCCACATATTATTTCCTGTGGTGCTCCAAAGTTGTGTCAGGGCTCTGCGGTGACCACAGCAGTCACTGTTTTCTGGCAGTCCAGTTATAAAAAGCTTTATTTCTGGAAATATGCAGAGCGGGTACATGTCCCTTTTTCTTCTTAATTAAGCTATGTAGAAGACACACACATACACATGCATATATATATATATGCATATATGTTTATTTCTGGGTTTAGAAATAGCTTCAGGAGTTCCTTCCAAGTCTCTGCTCTTCCTGGAGCTCTGCTTGCTGGTTGTTTTGGGGTGGTTTGTAGCTCTTGTTTCACTCCAATAACTGCCCACTAAGTTTCTGCCACAAGCAGGCATGGCTCTTAGTGTGTCTGCTTGGATCCTCATGGTTCTTGATTCCTTGCTTTGTCGTTTTTGCACACCTGTCTGAAATCCTCACTGAGGTGTGTTTCACTGTTTAAGGTGTAATGAAACTGCTGCAGAGTCCTTTATTAGTAGTGTTGTTGTGTGTGTGATGGATTTGCAAGCATATCCATCTTCTGGGCAAGGAAAAAGGGATTAAGAGTGTAATTTCTATTTCCTGTTCTTAAAAGTGATTATATTTTCTCTTCCACTTCAAGTACTCAGAGTGGTACAATTTTTGTTTTGGTGGGCATCCTTTCAAGAGCTTTCACTTAAGACTTTTTTTGCAGTGTAAGAATGTGCAGTGCTTACATCGTTCTACAGCTTGATGATGGTTCAGTTACTGACAAACACAGCACTGGAGTCGTTGTGCAGTGCAGCTGACTCTTGTCATATTCCACCTTCTTATTCTGTCTTGGTTTTTGTTCCCAAAGGTCTAATCTCTCCTCTGCATTTGTAAGTGGAAATTCAGATGTGATAACATTACAAAAAATCAGAGCTTATTGAGCCTTTTCTCAAAAAAGAAATGTGCTGTTAATGCTTAGAAAGCATTTTAAAAGATTCTGGGGGCTTTTTTGCTGTACTTATAACAGTTTCTTCATCCAGGGCATCTATAAGCTCTTCACTGTCTTTCCAGCTGAAATTTGCCTGTGGTCTCAGCTCTTGTGTACTTATTTTTTTGTGTATACCAACTAGTGCTGGTAACTATTGGAATAATCATGCAGTTGTAGTTCTTGGAAGTCCAGCACGGTCACACCGAATCAGGAATGCTTTTTGCTGGAGTTTTCCCTTCGATTCCTTTCTGTCCCCATTTTTGAAGTAAAATCTTCAGTCTCAGAGTAACTTATAAATACTTATTTCTAGAAATATAACCATATAGACAGCATAAGGAAGGGGAAGCACATAACAATATTATATATCTAACCTTATAGATAAACAGAACAAAAAGTTTATGATTATTATTCCATGGTATGTTTCTGTACTTGATATTTAAAGTTCTCTATGTGGAAGAGAGTTAAGGTCTTGGACTGCGATAAGCATCTTCAGTGTTAGAGTTTGTCAGGAATCTGTGTTCCACACTCTGTGAAGGCTAGCAAGCTTTCTGGGTTTCCTTTAAATGAGCCTTAAGATGTGTCAGTTAGCCAGCTAACTCGCTGGGTTTATTTTCCTGTTGTGACTAAAATGACATTTGTGCTCTGTTGTCAG
Seq C2 exon
ATGACGGGATCAAACGAGTTCAAACTCAATCAAACTCCAGATGATGGGATTTCATCGGTCAAGTTCAGTCCAAATACCTCTCAGTTTCTGCTTGTTTCATCCTGGGACACTACAGTCCGTCTCTATGATGTTCCTGCCAACAGCATGAGACTCAAATATCAACATTCAGGAGCTGTCCTTGACTGTGCTTTTTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000009681:ENSGALT00000015757:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.123
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0040027=WD40=WD(100=55.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGGACTCTCCGGGACCG
R:
AAAAAGCACAGTCAAGGACAGC
Band lengths:
302-2098
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]