MmuINT0026392 @ mm10
Intron Retention
Gene
ENSMUSG00000066979 | Bub3
Description
BUB3 mitotic checkpoint protein [Source:MGI Symbol;Acc:MGI:1343463]
Coordinates
chr7:131560222-131560904:+
Coord C1 exon
chr7:131560222-131560454
Coord A exon
chr7:131560455-131560709
Coord C2 exon
chr7:131560710-131560904
Length
255 bp
Sequences
Splice sites
5' ss Seq
GAGGTAGAG
5' ss Score
4.86
3' ss Seq
CTTAGCGTTTTGTTCTACAGATG
3' ss Score
9.76
Exon sequences
Seq C1 exon
GGGGGGGGGAAACGCGGCTTCCCTTTGAGCCGGAACAGGATGACTGCGGAAAATGAGGCAAGCAGTAAATCAGACCAATCAGCTGCCGAGATCGAGCCGTTGGCGTTCAAAATCAGCCAATGATAGGCCTGCTCCGGAGCTTCCTCTCCGCCTTCTCCGCTTTGCCTGCGAGTGCTCGGAGGGAAGCGGATCGGTAGTGGCCGGTGCAGAAACGTTGCTTAGGCGGACAGGAG
Seq A exon
GTAGAGGTTCGGGCGAGGGCGGGCGGAGCGGGATCTGTGGCCCGTGGCCGGCGCATCGATGCACAGAGGGATCCGGTCGAGGTCCATTGTGTTTTATATGGGGATCTGCGGGCAAGGAAGGAGGCCAGGCCGGGTGGAGCTCGGCGGAAGCGGGATGGCGGCCGGCCTGGATCAAGGGGGACTCGCGCAGGCGCAGGCCGTGGCCGGCCTCTGTTGCTCCGGTTAGGAACTCAGACTTAGCGTTTTGTTCTACAG
Seq C2 exon
ATGACCGGTTCGAACGAATTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCGGTTAAGTTCAGCCCCAACACATCCCAGTTCCTCCTGGTCTCCTCCTGGGATACGTCTGTGCGCCTCTACGATGTGCCCGCCAATTCCATGAGGCTCAAGTACCAGCACACTGGTGCTGTCCTGGACTGCGCCTTCTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000066979:ENSMUST00000084502:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
5' UTR
No structure available
Features
Disorder rate (Iupred):
C1=NA A=NA C2=0.246
Domain overlap (PFAM):
C1:
NA
A:
NA
C2:
PF0040027=WD40=WD(100=52.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGTTGGCGTTCAAAATCAGCC
R:
GTGTGCTGGTACTTGAGCCTC
Band lengths:
303-558
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types