DreINT0056588 @ danRer10
Intron Retention
Gene
ENSDARG00000076025 | dgkzb
Description
diacylglycerol kinase, zeta b [Source:ZFIN;Acc:ZDB-GENE-111104-4]
Coordinates
chr25:7698896-7699229:+
Coord C1 exon
chr25:7698896-7698991
Coord A exon
chr25:7698992-7699151
Coord C2 exon
chr25:7699152-7699229
Length
160 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGAC
5' ss Score
6.89
3' ss Seq
ATTTCGTTTTGTCCTCACAGCAA
3' ss Score
8.73
Exon sequences
Seq C1 exon
GAAAATGCCCAGTTGGGTCAACACGTGTGGTTTGAGACCAGTCCGGCAGGAGATTTCTGTTATGTGGGGGAGTCAAACTGTATCGCAAAGTCCCAG
Seq A exon
GTAGACACATATTGTAGTGATTCTTAAAACAATCATCAGCAAAAATGAGCAGTGTGTATCGAAAAACCCTGCACAACAGCTCCCTCTTCTGGTTAGATGTTCTATATGCATTTAGATAAATCGCTAAGATGCTAATTACCATTTCGTTTTGTCCTCACAG
Seq C2 exon
CAAAAGTCTCTTCCCCGCCACAAATGTGCAGCTTGCAAAATATCAGTTCACACAGAGTGCATGAATCAGCTGGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076025:ENSDART00000155016:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.031 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0013017=C1_1=PU(42.1=75.0)
A:
NA
C2:
PF0013017=C1_1=FE(43.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTTGGGTCAACACGTGTGG
R:
TGTCCAGCTGATTCATGCACT
Band lengths:
163-323
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]