DreINT0062408 @ danRer10
Intron Retention
Gene
ENSDARG00000056744 | ela2
Description
elastase 2 [Source:ZFIN;Acc:ZDB-GENE-041117-1]
Coordinates
chr8:21344205-21344493:+
Coord C1 exon
chr8:21344205-21344257
Coord A exon
chr8:21344258-21344404
Coord C2 exon
chr8:21344405-21344493
Length
147 bp
Sequences
Splice sites
5' ss Seq
GCGGTAAGA
5' ss Score
9.63
3' ss Seq
CATTTTATGTATTTTAACAGCCT
3' ss Score
7.1
Exon sequences
Seq C1 exon
ACTGCAATCAGGCACGATGAAACTTGTGATCTTGGCTCTGTTTATTGCTGGCG
Seq A exon
GTAAGATGGACGTTAAAAAAGGAGCTAAAAAAAACTGTTGTTCTTTTAATCCTCAGTAGTAGATTGAGGATAGATTTCACTTACAGTTTGCACTGAAGACAACGAACTTGAGCAGACAGTAATTTATCATTTTATGTATTTTAACAG
Seq C2 exon
CCTACGGTTGTGGGCAGCCCACCTACAAACCCATCGACTCGAGAGTGGTGGGAGGAAGTGATGTCAGACCCAACAGCTGGCCATGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000056744:ENSDART00000136765:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0008921=Trypsin=PU(6.0=46.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGCAATCAGGCACGATGAA
R:
CTGCCATGGCCAGCTGTTG
Band lengths:
142-289
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]