DreINT0063326 @ danRer10
Intron Retention
Gene
ENSDARG00000100666 | ep300a
Description
E1A binding protein p300 a [Source:ZFIN;Acc:ZDB-GENE-080403-16]
Coordinates
chr12:18824644-18825796:-
Coord C1 exon
chr12:18825613-18825796
Coord A exon
chr12:18824722-18825612
Coord C2 exon
chr12:18824644-18824721
Length
891 bp
Sequences
Splice sites
5' ss Seq
CTAGTGAGT
5' ss Score
7.43
3' ss Seq
CTTGTTTTGCTCCCTTGTAGATG
3' ss Score
10.7
Exon sequences
Seq C1 exon
GCGGAGTATTATCACTTTCTGGCAGAGAAGATCTATAAAATTCAGAAAGAACTGGAAGAGAAGCGAAGGACACGGTTACAGAAGCAGGGTATAATGCCCTCCCAGGCTGGCATGAACCCCTCTGGCCTGCAGCAAGCGACCGCTGGGATTGGTCAGCCTGGGCCACCCACAGGACTGCCTTCTA
Seq A exon
GTGAGTACTCAACCGCAGTGATTAAGACCCTGGTATACTTCAAACTAAATTTTGGAACTTGGAAACAGCTCAACATAGCAAACTTTTGTGGCAGTTCGTTTGGGGTTTTAAATATCTTTGAGCTACCAGTCAGTAAAAGATGTGTTTGTGTTTGGTAACTCTGTCTTCTTTGACATTTTACTTTACATTTAGTCATTTGACAGAAACTTTTGTCCAAAGCAACTTATAATTAAGGAGGAATTCAGCACGAAGAGGCAGTTCACACAGGAAGTCCTATTTATACAAAGGAACTGTTACTGCTCAGAGAACTAGGTGCTAGAGTAAGGGATGGGTTTTTTTGAGGAGGGAGTGAAGAGTGTTCGTACAAAGAGTGTTTGGGGATATTCTGAGAGGGTCACAAACCAGTTGAGAAGCCCACTTTATCATGCCACGTCTTTTCCATCAGGTTGTACACACTGGTGGCACCATTCGGTGTCTCTATGACAAGTGGAAATGATGTAAAGATGTGACTCTGTGGTGGTGCTTCTGGAGAAAGCCGTTTGCTAGATAATTTTTTTAATTGTTCTCTCTCAGGTGCCAACTTTGTTGAGTTTAGGGGTACATACAAGCAGCATGACACATGTACAGCCTTGTCTACTGCAACAAGGGTATGTGTTAGGGTTTAATACGGTATACCATCACTCAGCCTTTTCAAGCTTGCATTGTTTGTTTGAAGCATCTACACCTTTTAGCTACTTTTAAAGGAAGAGTTCACCCAATTTGGAATAGGTGTTGTTTTTTAAATTAAAAACATTTCACTGGGTCATAATAACAAGAACATGATGAGACTGAAAATGTATCAAGCAGTTTCATTGGGTTTGCTTGTTTATGTCTTGTTTTGCTCCCTTGTAG
Seq C2 exon
ATGGCCCACTATCAGATCCATCAGTAGTGCGTCCAACTGGCCCAAACCAGATGATGAACAGAATGCAGAATACTGCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100666:ENSDART00000172574:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.742 A=NA C2=0.903
Domain overlap (PFAM):
C1:
PF0217211=KIX=PD(23.5=30.6)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGGAGTATTATCACTTTCTGGCA
R:
TTCTGCATTCTGTTCATCATCTGGT
Band lengths:
254-1145
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]