DreINT0063350 @ danRer10
Intron Retention
Gene
ENSDARG00000100666 | ep300a
Description
E1A binding protein p300 a [Source:ZFIN;Acc:ZDB-GENE-080403-16]
Coordinates
chr12:18830844-18831346:-
Coord C1 exon
chr12:18831233-18831346
Coord A exon
chr12:18831051-18831232
Coord C2 exon
chr12:18830844-18831050
Length
182 bp
Sequences
Splice sites
5' ss Seq
AGAGTGAGT
5' ss Score
7.36
3' ss Seq
TGCTTCATAAAATATTTCAGCTA
3' ss Score
5.22
Exon sequences
Seq C1 exon
TGGCGCACTGTGCCTCATCCAGACAGATAATCTCTCACTGGAAGAACTGCACACGGCACGACTGCCCTGTGTGTCTGCCTTTGAAAAATGCAGGAGACAAGAGGAATCAGCAGA
Seq A exon
GTGAGTGTGTGTACTACTACAAAACATGCAGTGTTATACATCACCTATCTCAAACGTAGAACATATTTGAATCACTCCAATGTTGCAAGAAATTGTGCCTTTCAACAACCGTGTTAATTTATTCCAAATCAATGAAAACTGTCACTAATTTGGGTTTATATCTGCTTCATAAAATATTTCAG
Seq C2 exon
CTATGATAGCCACTGGAGGTGTGGCGTTAAGTTCTTCCATGGGCAATGTAACTGGTGGTTCACCCAGTGCCCCCAGTCTCAATACCCCAGGGCAGATAGACCCCAGCTCCATCGAGAGGGCATATGCAGCCTTGGGTCTCACATACCAGGGAAACCAGGCTCCCCCTCAACCTATACAGCAAACACAGCGGCCGGTGAACACCTTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100666:ENSDART00000172574:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.179 A=NA C2=0.814
Domain overlap (PFAM):
C1:
PF0213511=zf-TAZ=PD(32.1=66.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGTGCCTCATCCAGACAGA
R:
GTATGTGAGACCCAAGGCTGC
Band lengths:
254-436
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]