DreINT0093802 @ danRer10
Intron Retention
Gene
ENSDARG00000018460 | mbnl2
Description
muscleblind-like splicing regulator 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9582]
Coordinates
chr1:2053270-2053822:+
Coord C1 exon
chr1:2053270-2053323
Coord A exon
chr1:2053324-2053665
Coord C2 exon
chr1:2053666-2053822
Length
342 bp
Sequences
Splice sites
5' ss Seq
CTGGTACTA
5' ss Score
3.1
3' ss Seq
GTATGCTCTTTCACGCTCAGGCG
3' ss Score
6.79
Exon sequences
Seq C1 exon
ACTCAGTCGACTGCCAAAGCAATGAAGCGACCCCTCGAGGCATCTGTAGATCTG
Seq A exon
GTACTATGACCTTTCACCTTTTGCTTTGCATGTAGCTTTTTTAATGTACTGTAGCAGAACTCACAGAAAAAAAAAGTGATTTAATTTGGCTTCTTGCTTTTGATACAAACAGTAAAAAGTCACCAAGTGTACTTGTATGTAGAACTTACCATCAGTCGTAATTCTTGAAGCCTAGTAAACGTTAAGTGCCTTGCTAAAGTGTTTAGTGACGGTCGTCCTTGTGTTAAAAAAAAAAATAGACGGAGCCATTCAGCTTCAAGTCAACCTTGTGCATTGATTATTGTGTCAGACAATGCTTGCAAACCTGGTTTAACTGGTGCATGTATGCTCTTTCACGCTCAG
Seq C2 exon
GCGTTTCCGCATGGAGTCCTGCAGCCCCTACCAAAGAGACCAGCACTAGAAAAGAGTAACGGGGCCAGCACGCTCTTCAACCCCAGTGTCTTGCACTACCAACAGGCTTTGGCCAGCGCACAGCTTCAGCAGCCCACCGCTGCGTTCTTTCCCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000018460:ENSDART00000124534:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (disopred):
C1=1.000 A=NA C2=0.759
Domain overlap (PFAM):
C1:
PF149201=MTBP_C=FE(11.3=100)
A:
NA
C2:
PF149201=MTBP_C=PD(0.7=1.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTCAGTCGACTGCCAAAGC
R:
CTGTGGGAAAGAACGCAGCG
Band lengths:
211-553
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]