DreINT0095013 @ danRer10
Intron Retention
Gene
ENSDARG00000062686 | megf11
Description
multiple EGF-like-domains 11 [Source:ZFIN;Acc:ZDB-GENE-060503-252]
Coordinates
chr18:19251246-19252538:-
Coord C1 exon
chr18:19252402-19252538
Coord A exon
chr18:19251459-19252401
Coord C2 exon
chr18:19251246-19251458
Length
943 bp
Sequences
Splice sites
5' ss Seq
CAGGTTAAA
5' ss Score
2.54
3' ss Seq
AGTGTGTGAAATGTTTGCAGGTG
3' ss Score
6.09
Exon sequences
Seq C1 exon
GGTTCTGTGTGTGCCCAGCCATGTCCGTTTGGAAAATATGGGATCAACTGCAGCAAGGAATGCTCATGCCGCAATGGAGGACTCTGTGACCACATCACAGGACAGTGTCAGTGCATGGCGGGATACAGCGGCCACAG
Seq A exon
GTTAAACAGATCCCTGATCAGTTATTGATACTCTTTCGTGTCTTTAAAAAAAAAGTGGCACAAAATGCAATGTATGCAAAATGTAGGTACTGAATAATTGATTAATTGCTTTTTTACAACTCAGTGTTTACATTATGTCCACTGATCCCACTCCAAGGTGTGCACTGGTCTGTTAAGTTTCTGCCCATTAATTACCAGTAATAGAGTTTTATTAGGGTCCAATTGAAATTGCTCTTTATTTTCCAGTGTCTTCTGCCGCAGCTTAGGGTTTATTAGCGCGGTAAAGTGATTGTCTGCAGAATGAAAACACTTTCAGGACAGACACTTCTTATCCTCTAATATTTAACAAAATTGCCCTCATTCATTTAGTCTGTGACAGGTGAATTAGCCCAGCAGGAATAATCTCAGATTGCGTTAGTACTGCCGCCTCCCTTACCCCACGTCCTCCTTTCACCCGTCTCTCCTGATACAAACGACACCGGTGTAATTTCAACATGAAACATACCTAGTCCGTTTTATAATTTAATTGAGCTATTTATCTGTCTCCTAATGGCTTCTTTGGACACTGGAGATTGATGTTCTGCCAGGATTGTATATTGAAGATCTCGTGGAAGTCGTTGTTCCTTAAGTCACTTTGCTATTCAACAGAGAGAATGAGCCCGGGTCTAATTGTATTCGCGGTAATTAATGACTGCAGTCTGCGTTTTGCTGTCGTCCCATCTGTGAAGCCAAAGGGGCTGATGAACCTGAGGTACCACAGAAGATGCCCGGAGCTTTGACCCTCGACCCATAGATTAGAAAAGCTCTGTACTATACTAAATCTGCTTGTGCTATTTTGACAGCCTCTTTTTTTCTTTTAAGAATGTATGCTAGTGGTTTCATTGGGGTTTATGGGTAAATAGAGGGGTTTCTTCTTTCTCTGAAGTGTGTGAAATGTTTGCAG
Seq C2 exon
GTGCCAGGAAGAGTGTCCGGTTGGCACCTATGGACCCCAGTGCACCCTCCATTGTGACTGTCAGAATGGGGCAAAGTGTTACCACATCAATGGAGCTTGTTTGTGCGATACGGGATTCAAAGGGCACCACTGCCAGGATAGATTCTGTCCACCAGGCCTTTACGGTCTCATCTGTGACAAATACTGCCCCTGTAATTCCACCAACACAATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062686:ENSDART00000147902:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF126612=hEGF=PD(23.1=6.5),PF126612=hEGF=PU(84.6=23.9)
A:
NA
C2:
PF126612=hEGF=PD(7.7=1.4),PF0005319=Laminin_EGF=PU(18.8=12.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTCTGTGTGTGCCCAGCC
R:
TGTTGGTGGAATTACAGGGGC
Band lengths:
342-1285
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]