DreINT0101064 @ danRer10
Intron Retention
Gene
ENSDARG00000044632 | myo7ab
Description
myosin VIIAb [Source:ZFIN;Acc:ZDB-GENE-091110-4]
Coordinates
chr21:22202074-22202489:+
Coord C1 exon
chr21:22202074-22202189
Coord A exon
chr21:22202190-22202336
Coord C2 exon
chr21:22202337-22202489
Length
147 bp
Sequences
Splice sites
5' ss Seq
AAGGTGGGC
5' ss Score
7.93
3' ss Seq
ACAATATTCTCTTAATATAGGAG
3' ss Score
6.23
Exon sequences
Seq C1 exon
AGGGGGACTATGTCTGGTTGGACCTCAGAACAGACCATGAGTTTGACGTCCCTGTTGGTGCTGTGGTCAAATTTTGTGACTCGGGTCAAATCCAGGTCTTGGATGATGAAGGAAAG
Seq A exon
GTGGGCTTTTCACTGTTTACTAATCAATAATACAACTGACATGTGCAAAACAAAAACTTAAACTATAGATTCTGCTTAACTATTAATATGAATTAATATGAATATTGAACTTTGTCTTGGTTAATTAACAATATTCTCTTAATATAG
Seq C2 exon
GAGCAACAGATCTCTCTTCAGAATGCAACGAACATCAAGCCCATGCACCCAACCTCCATTCACGGGGTGGAGGATATGATCCGCCTAGGGGACCTGAATGAAGCTGGGATTCTTAGAAATCTCCTGATTCGTTACAATGACAGAGTCATATAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044632:ENSDART00000148019:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.077
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0006316=Myosin_head=PU(4.3=53.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGGGGACTATGTCTGGTTGG
R:
TCAGGAGATTTCTAAGAATCCCAGC
Band lengths:
243-390
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]