DreINT0114225 @ danRer10
Intron Retention
Gene
ENSDARG00000086927 | pik3c2b
Description
phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta [Source:ZFIN;Acc:ZDB-GENE-050309-145]
Coordinates
chr11:23365922-23366957:-
Coord C1 exon
chr11:23366777-23366957
Coord A exon
chr11:23366030-23366776
Coord C2 exon
chr11:23365922-23366029
Length
747 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGC
5' ss Score
7.16
3' ss Seq
GTCTTTGGTCTGCTGTTCAGGCT
3' ss Score
6.02
Exon sequences
Seq C1 exon
GTTGCTGAAAGACACTCTCCAGGACTCTCAGTTCAGCGTGAGGTATCAGTATCTGCTGGCGGCTCTGCTCTGCTGCTCCGGTCGTGGTTTAAGGGAAGAGTTTGATCGTCAGTGCTGGCTGGTCAATGTGCTGGCCAAAGTGGCTCAGCGGGTCAGAGAAGCGTCACCCTCATCCAGACAG
Seq A exon
GTCAGCTTCACTGTTGCTTTCTATATATTGTTGTGGTGTTTATTAAATTGTGCCATTATGGTTTTTTTTTACATTTATCTTTGTAGTGTGTAATTTAGCTGTTTGTGAGTGTAAAAGATCTGCAAAGATTTGCAGCTTAAAGTGTGAGTTATTGACTCTAAAAAGAAAGAACAGATTCAGAATTGAATGAAATGAGTTGATGGTATTTGTTTCCTGCACAAACCTATGTAAGATAATGACAAGATGTGTTGCTTTGATGTGTTATGAGAATATAGTCATATAATTATTTGTCTTTTTTCCCTTTTGATTAAAAAAAAAAAAAAAAACTATAGTTTTTTTATTAGTATATTATAAGATTCCTCAAACCTAATCTTTAAAATAAAATTTGGCCTCTTCAGTTTTTTTTTTTTTTTTAAATTCCATAATTTTTAAATATTTTTATTTTATTTTAATTCATCTCAGTTGGTTTGTCCAATTTTCATCCACTGTTTTTTTCCAGCTTTATAATTAAATTCACCTTAAAAATTGTGTAAACAGAGCCTTACAGGTTTTCTGATGCTCACTATTGTCTAAGAATCATCGGCTTTTCTCGTTTCATTAGACATCTCTATGGCTCTTACTATTTTTAGCTTGAAAGTCCAGATCTGTGCCAAAAAACTGATTGTTGATCCCTCAGGGACATTTTAGTGTTCTCCAGAGCGTCTGGCCAGCACATTAACAGCGTTTTGTCTTTGGTCTGCTGTTCAG
Seq C2 exon
GCTATTCTCCGCGAAGGGCTTGATGACGTAAAGCAGTTCTTCTCGATAAACAGCAGTTGTCGACTTCCGCTGAACCCAGGCCTGCTCGTCAAGGGCATCAACATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000086927:ENSDART00000124810:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061315=PI3Ka=PD(18.6=55.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTGAAAGACACTCTCCAGGA
R:
TGTATGTTGATGCCCTTGACGA
Band lengths:
286-1033
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]