GgaINT0132821 @ galGal4
Intron Retention
Gene
ENSGALG00000002225 | RFFL
Description
E3 ubiquitin-protein ligase rififylin [Source:RefSeq peptide;Acc:NP_001025882]
Coordinates
chr19:4499703-4502458:+
Coord C1 exon
chr19:4499703-4499726
Coord A exon
chr19:4499727-4499990
Coord C2 exon
chr19:4499991-4502458
Length
264 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAGT
5' ss Score
10.65
3' ss Seq
TGTCTCTCTTGTTTACATAGGTG
3' ss Score
10.52
Exon sequences
Seq C1 exon
TTTCCGACACTGATGATCAAACTG
Seq A exon
GTAAGTAATGTTCTACGTATTGCTTTATTTGTGTCATTGCTAAGAATCTCAGTGCTACCACATCCCTTTCCATTGTGCAGAAAACACTATGTAAAAGCTAAAGCTTCCTACTTCCCTGTTGATGGGAGCAACCATATGCCCACATGAATGTAGATGCAAAGGGGATTTTTCAGAGCTTCAGTGTGTGAATACATCAGAAGCTGTTGGATAAAGACAACTCACAGCCTGCACAAGAACATACCTTTGTCTCTCTTGTTTACATAG
Seq C2 exon
GTGGTGCTGGGCTCCCTGGTGTGGAGGACAACCTCTGCAAAATCTGCATGGACTTACCCATAGACTGCGTCCTGCTCGAGTGTGGCCACATGGTCACTTGCACCAAGTGCGGAAAGAGAATGAGTGAGTGTCCCATATGTAGGCAGTATGTGATAAGGGCTGTCCATGTCTTTAAGTCTTAAGTATGCATAAGGGAGACTGGTGATGCCTTAAAGCCTCATCTGCTGTTAGAGAAATGGGCAAAGTTGCTACAAAAAGTTAGGTCAGAGATTAAGATACAGGCCCTGCTGGGAGCAAGGGAAGGATGAAGCCTGGGGAATTGCCTACTCAAGCCATGTTTGCCTGTGCCCTTTCCACCATACTGTTTTAAACCACAGTGCCTGGACTCCCCGGAGCTTATTGCCAGCAGCAGACAAATAATTAGCAACCTCCATTCTAGAGTAACTGGATCCTCAGGTGGGAGACAAATGGATGTTTCTCACCATAGATCCTGAAGGTTCCTATTTTTCACTGAATAAAAAACACAGTAGACTACGGTCTCAAAAAGTGACATGTTTTGCTGCTATTGAATGTTGACTCTACTTCTGTTGTGTTGGGCAACACACCCACTGACAGCTTAGTTTTCAAGGAATAAAATTCTACTTTTTTTTTCCTAAATTTATTAGCTGCTTTTGCTTGCAGGACAGGCAGATTGCCTCCAATTTCCATCTTCAGAATAAGCTTAATTCTAGCCTAGTAGGATTATGGAGAAAATGTATTGTTTTATTTGAAGATGGGCTCTAGATTGCTGAAAAAAAAATGGGTAGTGAAAGACTGAAACATACAGCTTTACCTTGCCTGGTGTTCACTGTTTGTGCCAACAGGACAGGGACATACAGTCTGCTGCTGCTGGTTTGTTTGACTTTGTAGGTTTCCTTACAGTAGAGGTCAGCAGTCACTTCATGTCATTAAGGAGCTAAGAATCCACAGCTTGAGTATTTTAGATTTTGAGCTAGAGTAAGGTTTCTATTGCTACACAATCATAGTTAAGGATGGGCAACCTTAGAAGAGAGACCTGAAAGAAAGCAGAGGTGAACTTTATCTCCCTTGAATCAATAAAATGACAAGTGATCATCAGTGCAGAAACTTCTGTTTACTACAGCCCTGCTGCATTAAGACTGCTTAGCAAAAGTATGTATTTTGTGATCATTCTGCTTGTGAGGGTGGATAAGGTCTGTGGTATGACAGGTGACAATCACAAGCCCTTGGAGCTATCCTTGAAGAGTTTGTTCTCATGGAAGCTTTGCTAGAGAGAACAGATGGGCACGCAGTGGCCTGCAAGTGGCTGGTCCATTGGGCTCAAAGTTCCAGAATTTTGATGCCTTGAGTTTTCTGCCACTGTGGGATTGCATACTTGTTCCAAGTAACTTCTGAACTTGAGTACCTTCTACTGCAGGTTTTTCCTGCTCAACTTTCCCTTTTATCAGGTGCATCAAACTAAATCTTTGTGTGGGTGTCTAGTGTGGAATAGCATAAAACAAGTGTTGACTTAAAGCTTCTGGAGTAGTGGAAATGCAGTTACAGAAGTATCATTAAAGCCATCTACTATCTATTAAAGCAATGTTAGATAACAGCAAATCAACTTCTGCAATACTTAACATGCAGTCTTTAATGTTGCATAAGTGGCAACACACTCAGCTGGCACAGAGCAGAGGGGCTCTGCTTTAAAAGTAGGGATTATGGACTTAAGATGAAGACATCCATCATGCCACAGCTACCAGACTCTGTACTGCTGAGGTGGCCTCTGAACTTCCTGGTAAAAGCAGGACAGCTGTTGAGGCTTATTCAACTAAAAACACTGAGGTTTTCTTCCCCTGTTCAGGTTGTTCTCTAATACTTGGCTTGAGGTTTAACAGCTTCCAGCATGAGAACTGATACAATTCAATGCATCATGTTAAAAAAAAAAGTGCAGGTAAGCATTAACTATTTACTCTTCAGGAGATTCACAGGGAAATCTTTAAGAGATCAGAGTACACAAAGCTTTGAACAGTCTCATTAGTAAATTCACTGTGAGAATTCATGTCACTTTTCAATAACTCAGGACTGGGGGTATGTAGCAAGATCATTCCCCTGGGGGCAGATCTGCTTTTACAGGGTAGATTTATTTTCTATGGTAAATATATCAAGCTGCAAATCAATGGCAGAAATTCTATGAATGAGAAGAAGTACCCAGCTGAAGTCTGAAATGTGCTTTCTGAAGGGAAAAGCACCTTTTTCTGACTACACTGTTTTGCTTGTGTGAAATCAAAATCTGGAAGAACTGTTTAATAATCCAAAGGAATATAGTGATTTGAACTAATAAAGTAAATGGAAATATTTATGTGCTTTATGTACACTGTACATAGTTTAAATATTTTAAAGTTAATATTCTACTTGAAATGGCATGTTATTCTCTATTAAATCCTCTTGGTACTACAATAGTTAAACTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000002225:ENSGALT00000003484:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.556 A=NA C2=0.017
Domain overlap (PFAM):
C1:
PF102084=Armet=PD(6.5=55.6)
A:
NA
C2:
PF139201=zf-C3HC4_3=WD(100=75.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCGACACTGATGATCAAACTG
R:
GGGCCTGTATCTTAATCTCTGACC
Band lengths:
307-571
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]