MmuEX0027455 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123491694-123493255:-
Coord C1 exon
chr4:123493086-123493255
Coord A exon
chr4:123492641-123492819
Coord C2 exon
chr4:123491694-123491832
Length
179 bp
Sequences
Splice sites
3' ss Seq
TGTGCATTTTGTTCTGATAGCAC
3' ss Score
6.3
5' ss Seq
TAAGTGAGT
5' ss Score
6.43
Exon sequences
Seq C1 exon
AGGACAAAGAAGAGACTTTGGCCAAGGTATACATCTCTGAGCTAAAGAATATCCGACTACTCCTGGAGGAGTGTGAGCAGAGACTGCTCAAACAAATTCAGTCTCCAGCCAGCTCCAAGACTGACAGAGATGCCAGGCAGGACATCACATTGAGGATTGCAGAGCAGGAG
Seq A exon
CACACACAGGAGGATTTGCAGCATTTGAGATCTGACCTGGATGCTATTTCTATGAAATGCAACGTCTTTCTCCAGCAGTCTCCATCAGGTTCAAGTGCCACAACTCTGCGCTCAGAGCTGAACCTGATGGTAGAGAAGATGGACCATGTCTATGGTCTGTCTACTGTCTATCTGAATAA
Seq C2 exon
GTTAAAGACAATTGATGTTATAGTCCGTAGCATGCAGGATGCTGAGCTGTTGGTCAAAGGTTATGAGATCAAGCTGAGTCAAGAGGAAGCAGTGCCAGCAGACCTCTCAGCTCTGGAGTCCCATCGGACCACCTTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_CASSETTE8
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.316 A=0.100 C2=0.064
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGGAGTGTGAGCAGAGACT
R:
CTGTAAGGTGGTCCGATGGGA
Band lengths:
245-424
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types