BtaEX6018660 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107318312-107320769:-
Coord C1 exon
chr3:107320600-107320769
Coord A exon
chr3:107319142-107319320
Coord C2 exon
chr3:107318312-107318450
Length
179 bp
Sequences
Splice sites
3' ss Seq
GTGTGTCTTGTTCCTGATAGCAC
3' ss Score
7.55
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
Exon sequences
Seq C1 exon
AGGACAAAGAGGAAACTGTGGCCAAGATGTACATTTCAGAGCTGAAGAACATCCGGCTGCGCCTGGAGGAGTGTGAACAGAGGCTAATCCAGCGAATTCAGTCCCCAGCCAGCTCTCGGACTGACAAAGATGCTCGGCAAGACAACGCATTAAGGATTGCAGAACAAGAG
Seq A exon
CACACTCAAGCGGATCTGCAGCAATTGAGGGCAGACTTGGATGCTGTTTCCATGAAATGCAACAGCTTTCTCCATCAGTCCCCATCTGGTTCCAGTGTCCCAACTCTGCGCTCAGAACTCAATCTGCTGGTGGAGAAAATGGACCATGTGTATGGTCTCTCCACTGTCTATTTAAACAA
Seq C2 exon
GCTGAAGACAATCGATGTTATAGTGCGTAGCATCCAGGATGCTGAACTCTTGGTCAAAGGTTATGAAATCAAGCTGAGTCAAGAAGAAGCAGTACCACCAGATCTTTCAGCTCTGGAGTCCCATCGGTCTACATTACGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'29-31,'29-30,30-31=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.404 A=0.019 C2=0.071
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCTGAAGAACATCCGGCTG
R:
TGGGACTCCAGAGCTGAAAGA
Band lengths:
255-434
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]