RnoEX6023199 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141174872-141176425:-
Coord C1 exon
chr5:141176256-141176425
Coord A exon
chr5:141175810-141175988
Coord C2 exon
chr5:141174872-141175010
Length
179 bp
Sequences
Splice sites
3' ss Seq
TGTGCGTTTGTTCCTGATAGCAC
3' ss Score
7.28
5' ss Seq
TAAGTGAGT
5' ss Score
6.43
Exon sequences
Seq C1 exon
AGGACAAAGAAGAGACTTTGGCAAAGGTATACATCTCAGAGCTGAAGAATATCCGACTTCGCCTGGAGGAATGTGAGCAGAGGCTGCTCAAACAAATTCAGTCTTCAGCCAGCTCTAAGACTGACAGAGATGCCAGGCAGGACGTCGCATTGAGGATTGCAGAGCAGGAG
Seq A exon
CACGTGCAGGAGGATTTGAAGCATTTGAGATCTGACCTGGATGCTGTTTCTGTGAAATGCACCACCTTTCTCCAGCAGTCTCCATCAGGCTCGAGTGCCACAACTCTGCGCTCCGAGCTGAACCTAATGGTCGAGAAGATGGACCATGTCTATGGTCTGTCTATTGTCTGTCTGAATAA
Seq C2 exon
GTTAAAGACAATTGATGTTATAGTACGCAGCATACAGGATGCTGAACTGTTGGTCAAAGGTTATGAGATCAAGCTGAGTCAAGAAGAAGCGGTGCCAGCAGACCTCTCAGCTCTGGAGTCCCATCGAACCACATTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'48-45,'48-43,49-45=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.053 A=0.033 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATATCCGACTTCGCCTGGAGG
R:
GGACTCCAGAGCTGAGAGGTC
Band lengths:
243-422
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]