Special

MmuEX0027457 @ mm10

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123486667-123489372:-
Coord C1 exon
chr4:123489210-123489372
Coord A exon
chr4:123488329-123488412
Coord C2 exon
chr4:123486667-123486774
Length
84 bp
Sequences
Splice sites
3' ss Seq
TGTGATACTTTGGTTTCTAGGAA
3' ss Score
8.66
5' ss Seq
AAGGTAATT
5' ss Score
8.83
Exon sequences
Seq C1 exon
GCAATCTGAGGTAGAAAGTATCCAGGAAGTTCTGAGAGATTACCGAGCCTGCCATGGAACTCTCATCAAGTGGATTGAGGAAACCACTGCCCAGCAGGAAATGATGAAGCCTGGCCAGGCAGAGGATAGTAGAGTGCTGTCAGAGCAGCTTAGTCAGCAGACG
Seq A exon
GAACTCTTTGCTGAAATTGAGAGAAATCAGACAAAGTTGGACCAATGTCAAAAATTTTCCCAGCAGTACTCCACTATTGTGAAG
Seq C2 exon
GACTATGAGTTGCAGCTTATGACATATAAAGCCTTCGTGGAGTCACAGCAGAAATCTCCAGGCAAGCGCCGCCGCATGATTTCCTCTTCAGATGCCATCACACAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'64-56,'64-55,65-56
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.273 A=0.000 C2=0.278
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000080755





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000081324, ENSMUSP00000095507, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000114568, ENSMUSP00000115847, ENSMUSP00000119885, ENSMUSP00000123246
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr1:39319664-39319747 
HIGH PSI
HsaEX0037165
(MACF1)
Human
(hg19)
chr1:39785336-39785419 
HIGH PSI
HsaEX0037165
(MACF1)
Rat
(rn6)
chr5:141171789-141171872 
HIGH PSI
RnoEX6023202
(Macf1)
Cow
(bosTau6)
chr3:107315500-107315583 
HIGH PSI
BtaEX6018664
(MACF1)
Chicken
(galGal4)
chr23:5336644-5336727 
HIGH PSI
GgaEX6025273
([1])
Chicken
(galGal3)
chr23:5651820-5651903 
HIGH PSI
GgaEX6025273
(MACF1)
Zebrafish
(danRer10)
chr19:35570286-35570369 
HIGH PSI
DreEX6073699
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCCTGCCATGGAACTCTCATC

				
R: 
TGCCTGGAGATTTCTGCTGTG

				
Band lengths: 
181-265

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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