Special

MmuEX0027467 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123045118-123047211:-
Coord C1 exon
chr4:123047048-123047211
Coord A exon
chr4:123046064-123046075
Coord C2 exon
chr4:123045118-123045280
Length
12 bp
Sequences
Splice sites
3' ss Seq
ATTTGTGCATATCAAAGCAGCTA
3' ss Score
1.67
5' ss Seq
GAAGTGAGT
5' ss Score
7.97
Exon sequences
Seq C1 exon
GAAAGTCCTTGAATCAACCCACACCTCCCCCCATGCCAATCCTCTCACAGTCTGAAGCAAAAAACCCACGAATCAACCAGCTCTCTGCCCGGTGGCAGCAGGTGTGGCTGTTAGCCCTGGAGCGTCAGCGGAAGCTGAATGATGCCTTGGACAGACTGGAGGAG
Seq A exon
CTATGCCCGGAA
Seq C2 exon
TTGAAAGAATTTGCCAACTTTGACTTTGATGTCTGGAGGAAAAAGTATATGCGTTGGATGAATCATAAAAAATCTCGAGTCATGGATTTCTTCCGGCGTATTGACAAGGACCAGGATGGGAAGATAACACGTCAGGAGTTTATCGATGGCATTTTAGCATCTA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-MICROEX1
Average complexity
MIC_S
Mappability confidence:
NA
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.375 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF134991=EF-hand_7=PU(7.4=16.3)

							
A:
NO

							
C2:
PF134991=EF-hand_7=PU(54.2=70.9)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000081324f2416A





     
                   









    
Main Skipping Isoform:
ENSMUSP00000101819





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000080755, ENSMUSP00000081324, ENSMUSP00000081324f6186A, ENSMUSP00000095508, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000101831f2417A, ENSMUSP00000117188
          









    
Other Skipping Isoforms:
ENSMUSP00000095509, ENSMUSP00000114568, ENSMUSP00000119600, ENSMUSP00000119885
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39459821-39459832 
ALTERNATIVE
HsaEX0037158
(MACF1)
Human
(hg19)
chr1:39925490-39925504 
ALTERNATIVE
HsaEX0037158
(MACF1)
Rat
(rn6)
chr5:141057866-141057877 
ALTERNATIVE
RnoEX0051768
(Macf1)
Cow
(bosTau6)
chr3:107200778-107200789 
ALTERNATIVE
BtaEX0021012
(MACF1)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
chr23:5712355-5712369 
ALTERNATIVE
GgaEX0029075
(MACF1)
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CAGCAGGTGTGGCTGTTAGC

				
R: 
CCAGACATCAAAGTCAAAGTTGGC

				
Band lengths: 
105-117

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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