Special

MmuEX0027469 @ mm10

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123385455-123387431:-
Coord C1 exon
chr4:123387228-123387431
Coord A exon
chr4:123386549-123386557
Coord C2 exon
chr4:123385455-123385686
Length
9 bp
Sequences
Splice sites
3' ss Seq
GACTGCGCCCACCATTGCAGATC
3' ss Score
8.21
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
Exon sequences
Seq C1 exon
CAACTAAGGGAATCCATTGCTGAACACAAACCTCATATTGATAAGATACTAAAAATAGGTCCACAACTAAAGGAGCTAAACCCTGAGGAAGGGAAAATGGTTGAAGAAAAATACCAGAAAGCAGAAAACATGTATGCCCAAATAAAAGATGAGGTGCGCCAGCGTGCCCTGGCTCTGGATGAGGCTGTGTCCCAGTCTGCTCAG
Seq A exon
ATCACAGAG
Seq C2 exon
TTTCATGATAAAATTGAACCCATGTTGGAGACCCTGGAGAACCTGTCCTCTCGCCTGCGAATGCCTCCCCTGATTCCTGCTGAAGTCGACAAGATCAGAGAGTGCATCAGTGACAATAAAAGTGCCACCGTGGAGCTGGAGAAACTGCAGCCGTCCTTTGAAGCCCTGAAGCGGCGGGGAGAGGAACTCATTGGAAGATCGCAGGGAGCAGACAAGGATCTGGCTGCAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-MICROEX4
Average complexity
MIC-S
Mappability confidence:
NA
Protein Impact





	

	
Alternative protein isoforms (No Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.279 A=0.000 C2=0.077
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0043516=Spectrin=PD(56.6=88.2),PF060097=Laminin_II=PU(52.4=80.9),PF0043516=Spectrin=PU(0.9=1.5)

							
A:
PF060097=Laminin_II=FE(1.9=100),PF0043516=Spectrin=FE(1.8=100)

							
C2:
PF060097=Laminin_II=PD(42.9=57.7),PF0043516=Spectrin=FE(69.4=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000117433





     
                   









    
Main Skipping Isoform:
ENSMUSP00000080755





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000081324, ENSMUSP00000095507, ENSMUSP00000101819, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000114568, ENSMUSP00000119885
  



Associated events







Other assemblies


mm9




Conservation


Human
(hg38)
chr1:39436482-39436490 
ALTERNATIVE
HsaEX0037157
(MACF1)
Human
(hg19)
chr1:39902154-39902162 
ALTERNATIVE
HsaEX0037157
(MACF1)
Rat
(rn6)
chr5:141075461-141075469 
ALTERNATIVE
RnoEX0051762
(Macf1)
Cow
(bosTau6)
chr3:107220368-107220376 
ALTERNATIVE
BtaEX0021013
(MACF1)
Chicken
(galGal4)
chr23:5383532-5383540 
ALTERNATIVE
GgaEX0004832
([1])
Chicken
(galGal3)
chr23:5700879-5700887 
ALTERNATIVE
GgaEX0004832
(MACF1)
Zebrafish
(danRer10)
chr19:35486705-35486713 
ALTERNATIVE
DreEX0044251
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TAAAAGATGAGGTGCGCCAGC

				
R: 
AGGGTCTCCAACATGGGTTCA

				
Band lengths: 
97-106

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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