BtaEX0021013 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107219199-107221295:-
Coord C1 exon
chr3:107221088-107221295
Coord A exon
chr3:107220368-107220376
Coord C2 exon
chr3:107219199-107219430
Length
9 bp
Sequences
Splice sites
3' ss Seq
GACTGCGCCCACCATTACAGATT
3' ss Score
6.74
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
Exon sequences
Seq C1 exon
CAACTGAGGGAATCCATTGCTGAACACAAACCTCATATTGATAAACTGCTAAAGATAGGCCCTCAACTGAAGGAGCTAAACCCTGAAGAGGGGCAAATGGTGGAAGAAAAATACCAGAAAGCAGAAAACACATATGCCCAAATAAAAGAGGAGGTGCGCCAGCGGGCCCTGGCTCTGGACGAGGCTATTTCCCAGTCGGCTCAGGTAT
Seq A exon
ATTACAGAG
Seq C2 exon
TTCCATGATAAAATTGAGCCCATGTTGGAAACTCTGGAGAGTCTTTCCTCTCGCCTGCGAATGCCACCGCTGATTCCTGCTGAAGTGGACAAGATCAGAGAGTGCATCAGCGAGAACAAGAGTGCCACCGTGGAGCTGGAAAAACTGCAGCCATCCTTCGAGGCCCTGAAGCGCCGTGGAGAAGAACTCATTGGGCGATCTCAAGGAGCTGACAAGGATCTGGCTGCAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-MICROEX2-1/2
Average complexity
MIC_M
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.338 A=0.000 C2=0.042
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(56.6=88.2),PF060097=Laminin_II=PU(52.0=77.9),PF0043516=Spectrin=PU(0.9=1.5)
A:
PF060097=Laminin_II=FE(2.0=100),PF0043516=Spectrin=FE(1.8=100)
C2:
PF060097=Laminin_II=PD(43.1=56.4),PF0043516=Spectrin=FE(69.4=100)
Main Inclusion Isoform:
ENSBTAT00000033270fB4005
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTCCCAGTCGGCTCAGGTAT
R:
CTTCAGCAGGAATCAGCGGTG
Band lengths:
106-115
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development