MmuEX0027471 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123360910-123365347:-
Coord C1 exon
chr4:123365273-123365347
Coord A exon
chr4:123364057-123364074
Coord C2 exon
chr4:123360910-123361027
Length
18 bp
Sequences
Splice sites
3' ss Seq
TTTACTCTTACTGTCTATAGTTC
3' ss Score
6.26
5' ss Seq
CAGGTACAG
5' ss Score
8.68
Exon sequences
Seq C1 exon
GTCACAAGACAAGTGGCTCAGTGCAAGTGTGCAAAAAGATTCCAAGTGGAACAGATTGGAGAGAATAAATACCGG
Seq A exon
TTCTTCCTCGGCAATCAG
Seq C2 exon
TTTGGAGATTCTCAGCAGTTGCGGCTGGTCCGTATTCTGCGCAGCACTGTGATGGTCCGAGTTGGTGGAGGATGGATGGCCCTGGACGAGTTTCTAGTGAAAAATGACCCCTGCCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'175-182,'175-180,177-182
Average complexity
S
Mappability confidence:
100%=100=80%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.002
Domain overlap (PFAM):
C1:
PF0218712=GAS2=FE(30.4=100)
A:
PF0218712=GAS2=FE(6.3=100)
C2:
PF0218712=GAS2=FE(49.4=100)
Other Inclusion Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGTGGCTCAGTGCAAGTGTG
R:
TCACAGTGCTGCGCAGAATAC
Band lengths:
117-135
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types