Special

MmuEX0027471 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123038153-123042590:-
Coord C1 exon
chr4:123042516-123042590
Coord A exon
chr4:123041300-123041317
Coord C2 exon
chr4:123038153-123038270
Length
18 bp
Sequences
Splice sites
3' ss Seq
TTTACTCTTACTGTCTATAGTTC
3' ss Score
6.26
5' ss Seq
CAGGTACAG
5' ss Score
8.68
Exon sequences
Seq C1 exon
GTCACAAGACAAGTGGCTCAGTGCAAGTGTGCAAAAAGATTCCAAGTGGAACAGATTGGAGAGAATAAATACCGG
Seq A exon
TTCTTCCTCGGCAATCAG
Seq C2 exon
TTTGGAGATTCTCAGCAGTTGCGGCTGGTCCGTATTCTGCGCAGCACTGTGATGGTCCGAGTTGGTGGAGGATGGATGGCCCTGGACGAGTTTCTAGTGAAAAATGACCCCTGCCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (No Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.002
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0218712=GAS2=FE(30.4=100)

							
A:
PF0218712=GAS2=FE(6.3=100)

							
C2:
PF0218712=GAS2=FE(53.2=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000101831f2417A





     
                   









    
Main Skipping Isoform:
ENSMUSP00000095509





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000076290, ENSMUSP00000080755, ENSMUSP00000095508, ENSMUSP00000101819, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000117188, ENSMUSP00000119600
          









    
Other Skipping Isoforms:
ENSMUSP00000081324, ENSMUSP00000081324f2416A, ENSMUSP00000081324f6186A, ENSMUSP00000114568, ENSMUSP00000116085, ENSMUSP00000119885
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39465095-39465112 
ALTERNATIVE
HsaEX0037169
(MACF1)
Human
(hg19)
chr1:39930767-39930784 
ALTERNATIVE
HsaEX0037169
(MACF1)
Rat
(rn6)
chr5:141053236-141053253 
ALTERNATIVE
RnoEX0051767
(Macf1)
Cow
(bosTau6)
chr3:107195616-107195633 
ALTERNATIVE
BtaEX0021017
(MACF1)
Chicken
(galGal4)
chr23:5401406-5401423 
Chicken
(galGal3)
chr23:5714256-5714273 
ALTERNATIVE
GgaEX0004823
(MACF1)
Zebrafish
(danRer10)
chr19:35467591-35467608 
ALTERNATIVE
DreEX0044253
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AAGTGGCTCAGTGCAAGTGTG

				
R: 
TCACAGTGCTGCGCAGAATAC

				
Band lengths: 
117-135

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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