MmuEX0027473 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123440550-123444952:-
Coord C1 exon
chr4:123444835-123444952
Coord A exon
chr4:123441603-123441665
Coord C2 exon
chr4:123440550-123440791
Length
63 bp
Sequences
Splice sites
3' ss Seq
CTCCCTGTCCTTCTGCTCAGGTT
3' ss Score
10.46
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
Exon sequences
Seq C1 exon
GATCTTATATCTGACTGGGAAAGTAAAGGAGCCCTGCTGGGAGAAATCAACGCCAAGGGCACTGCCTTAGAAAGTCTCATCATGGACATCACAGCTCCTGATTCCCAAGCCAAAACAG
Seq A exon
GTTCCATACTACCCCCTGTAGGAAGCTCTGTAGGCAGTGTAAACGGATACCACACCTGCAAAG
Seq C2 exon
ATCTTACAGAGATTCAGTGTGACATGTTTGATGTAAATTCGAAGTATGAGAAACTGTGGGAAGTGCTGCGAGAACGACAGGAAAGCCTTCAGACTGTCTTCAGCAGAATGGAGGAGGTTCAAAAGGAGGCGAGCTCTGTATTACAGTGGTTAGAATCAAAAGAAGAAGTCCTGAAAGCCATGGATGCCACTTTGTCTCCAACCAAGACAGAAACAGTAAAAGCCCAAGCAGAATCTAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_CASSETTE5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.071 A=0.273 C2=0.197
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(33.9=100)
A:
NO
C2:
PF0043516=Spectrin=PD(27.0=38.3),PF0043516=Spectrin=PU(41.8=56.8)
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACAGCTCCTGATTCCCAAGC
R:
TGCTGAAGACAGTCTGAAGGC
Band lengths:
134-197
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types