DreEX0044256 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35536290-35541165:-
Coord C1 exon
chr19:35541054-35541165
Coord A exon
chr19:35539267-35539329
Coord C2 exon
chr19:35536290-35536528
Length
63 bp
Sequences
Splice sites
3' ss Seq
TTTGTGTGTCTTTCTCCCAGGTG
3' ss Score
11.75
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GATCTGCTCACGGAATGGGAGACCCAAGGAAACCAAGTTCAGGAGTTAAACAAGAACAGTTCTCAGCTGGAGAACTTGATTATTAACACCACAGCACCCAAGAACAAGACTG
Seq A exon
GTGTTTCCCAGCTCAATGGTGCCTCCAGCCCCAACAGTGTTAATGGCATTCACACCTGTAAAG
Seq C2 exon
ACTTGACGGAGATCCAGGTAGCAGTGGCAGATGTGAACTGCAAGTATGAGCAGCTGGGCAGTGACCTGAGGGAGAGGAAGAGCAAACAAGAGTCTTCTCTAGAGCTGCAGCAGAAGGCCAGGCAGAGCACTGAAGCCCTCATCCAGTGGCTCGGCCCCCGTGAACAGAGCCTGGCACAAGGGCAGACCGCCTCACCTTCTCGGCCAGAGGTGGTGCGTGCTCAAGCACAACAGACCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533_CASSETTE5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.553 A=0.773 C2=0.562
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(41.6=100)
A:
PF0043516=Spectrin=PD(7.9=31.8)
C2:
PF0043516=Spectrin=PU(41.3=56.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCTGCTCACGGAATGGGAGA
R:
CACATCTGCCACTGCTACCTG
Band lengths:
146-209
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]