MmuEX0027473 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123117793-123122195:-
Coord C1 exon
chr4:123122078-123122195
Coord A exon
chr4:123118846-123118908
Coord C2 exon
chr4:123117793-123118034
Length
63 bp
Sequences
Splice sites
3' ss Seq
CTCCCTGTCCTTCTGCTCAGGTT
3' ss Score
10.46
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
Exon sequences
Seq C1 exon
GATCTTATATCTGACTGGGAAAGTAAAGGAGCCCTGCTGGGAGAAATCAACGCCAAGGGCACTGCCTTAGAAAGTCTCATCATGGACATCACAGCTCCTGATTCCCAAGCCAAAACAG
Seq A exon
GTTCCATACTACCCCCTGTAGGAAGCTCTGTAGGCAGTGTAAACGGATACCACACCTGCAAAG
Seq C2 exon
ATCTTACAGAGATTCAGTGTGACATGTTTGATGTAAATTCGAAGTATGAGAAACTGTGGGAAGTGCTGCGAGAACGACAGGAAAGCCTTCAGACTGTCTTCAGCAGAATGGAGGAGGTTCAAAAGGAGGCGAGCTCTGTATTACAGTGGTTAGAATCAAAAGAAGAAGTCCTGAAAGCCATGGATGCCACTTTGTCTCCAACCAAGACAGAAACAGTAAAAGCCCAAGCAGAATCTAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_CASSETTE6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.067 A=0.273 C2=0.196
Domain overlap (PFAM):
C1:
PF060097=Laminin_II=PD(17.5=42.5),PF0043516=Spectrin=FE(33.9=100)
A:
NO
C2:
PF0043516=Spectrin=PD(27.0=38.3),PF0043516=Spectrin=PU(41.8=56.8)
Other Inclusion Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACAGCTCCTGATTCCCAAGC
R:
TGCTGAAGACAGTCTGAAGGC
Band lengths:
134-197
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: