Special

MmuEX0027694 @ mm9

Exon Skipping

Gene
ENSMUSG00000020623 | Map2k6
Description
mitogen-activated protein kinase kinase 6 [Source:MGI Symbol;Acc:MGI:1346870]
Coordinates
chr11:110260436-110351507:+
Coord C1 exon
chr11:110260436-110260739
Coord A exon
chr11:110343853-110343919
Coord C2 exon
chr11:110351459-110351507
Length
67 bp
Sequences
Splice sites
3' ss Seq
CCTTATGTCCTTCCCCGTAGGCA
3' ss Score
12.26
5' ss Seq
CACGTAAGT
5' ss Score
10.16
Exon sequences
Seq C1 exon
AGTTCCAAGTTTGGAGCTTTTAGCTGCCAGCCCTGGCCCATCATGTAGCTGCAGCACAGCCTTCCCTAACGTTGCAACTGGGGGAAAAATCACTTTCTAGACTGTTTTGCAAGGTGTGCAATTCCACCTTGACTCCCTGAAAGTCCATCTGCTGCATCGGTCAAGAGAAACTCCACTTGCATGAAGATTGCACGCCTGCAGCTTGCATCTTTGTTGCAAAACTAGCTACAGAAGAGAAGCAAGGCAAAGTCTTTTGTGCTCCCCTCCCCCATCAAAGGAAAGGGGAAAATGTCTCAGTCGAAAG
Seq A exon
GCAAGAAGCGAAACCCGGGCCTTAAGATTCCAAAAGAAGCGTTTGAACAGCCTCAGACCAGTTCCAC
Seq C2 exon
GCCGCCTCGGGATTTAGACTCCAAGGCTTGCATATCTATTGGAAACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020623-'0-8,'0-6,5-8
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=1.000 A=0.913 C2=0.471
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000020949





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000020949f791A, ENSMUSP00000097831
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr17:69505780-69505846 
HIGH PSI
HsaEX6024519
(MAP2K6)
Human
(hg19)
chr17:67501921-67501987 
HIGH PSI
HsaEX6024519
(MAP2K6)
Rat
(rn6)
chr10:98782950-98783016 
HIGH PSI
RnoEX0052215
(Map2k6)
Cow
(bosTau6)
chr19:61743634-61743700 
HIGH PSI
BtaEX0021220
(MAP2K6)
Chicken
(galGal4)
chr18:8279233-8279299 
HIGH PSI
GgaEX1034809
(MAP2K6)
Chicken
(galGal3)
chr18:8016177-8016243 
Zebrafish
(danRer10)
chr12:36441463-36441532 
HIGH PSI
DreEX6070553
(map2k6)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CATCTGCTGCATCGGTCAAGA

				
R: 
TGGAGTCTAAATCCCGAGGCG

				
Band lengths: 
182-249

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 1 annotated functions for this event 


PMID: 26538579
HsaEX6024519
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: bimolecular fluorescence complementation, filter binding. ELM ID: ELMI003289; ELM sequence: KKRNPGLKI; Overlap: FULL


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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