Special

MmuEX0053641 @ mm9

Exon Skipping

Gene
ENSMUSG00000031728 | Zfp821
Description
zinc finger protein 821 [Source:MGI Symbol;Acc:MGI:1923121]
Coordinates
chr8:112233311-112245243:+
Coord C1 exon
chr8:112233311-112233427
Coord A exon
chr8:112241662-112241787
Coord C2 exon
chr8:112245095-112245243
Length
126 bp
Sequences
Splice sites
3' ss Seq
CTTTTTCTTGTGATCACCAGGGG
3' ss Score
7.91
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
GTCAGCACCGTACGAGGAGGTAGTGACCTGTCCAGGGGTGAAACTGGGGAAGAACGGAGCTTGGATATCAGGGAAACATGTCCCGTAGGAAACAGACAAACCCAAATAAAGTTCACT
Seq A exon
GGGATCAAGTCTTTGCTGGGTTAGAAGAACAAGCCCGACAGGCAATGATGAAAACCGATTTTCCTGGAGACCTTGGCAGTCAGCGACAAGCTATCCAACAACTACGAGATCAGGACTCCAGTAGCA
Seq C2 exon
GTGACAGTGAAGGTGACGAAGAGGAGACCACACAAGATGAAGTATCTTCCCACACATCAGAGGAAGATGGTGGGGTAGTCAAAGTGGAAAAGGAATTAGAGACTGCGGAAGCACCTGTCAGTAGTGGGGACGCACTAGCAGAGCGCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031728-'4-6,'4-5,6-6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=1.000 A=1.000 C2=1.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000034163





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000126195
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr16:71867912-71868037 
ALTERNATIVE
HsaEX0074408
(ZNF821)
Human
(hg19)
chr16:71901815-71901940 
ALTERNATIVE
HsaEX0074408
(ZNF821)
Rat
(rn6)
chr19:41947436-41947561 
HIGH PSI
RnoEX0101712
(Zfp821)
Cow
(bosTau6)
chr18:39362125-39362250 
ALTERNATIVE
BtaEX0041563
(ZNF821)
Chicken
(galGal4)
chr11:19181694-19181819 
ALTERNATIVE
GgaEX0000801
(ZNF821)
Chicken
(galGal3)
chr11:21709563-21709688 
ALTERNATIVE
GgaEX0000801
(ZNF821)
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ACCGTACGAGGAGGTAGTGAC

				
R: 
CTAGTGCGTCCCCACTACTGA

				
Band lengths: 
249-375

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 1 annotated functions for this event 


PMID: 31911676
HsaEX0074408
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.846, FDR=0.038), PC9 No Change at 14 days (FC=0.953, FDR=0.584), Late Xenograft Enriched (FC=1.741, FDR=0.002).


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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