RnoEX0101712 @ rn6
Exon Skipping
Gene
ENSRNOG00000000262 | Zfp821
Description
zinc finger protein 821 [Source:RGD Symbol;Acc:1306674]
Coordinates
chr19:41944450-41955509:-
Coord C1 exon
chr19:41955393-41955509
Coord A exon
chr19:41947436-41947561
Coord C2 exon
chr19:41944450-41944598
Length
126 bp
Sequences
Splice sites
3' ss Seq
GTTTTTCTTGTGACCACCAGGGG
3' ss Score
6.94
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
GTCAGCACCTTACAAGGAGGTAGTGACCTGTCCAGGGGTGAAACTGGGAAAGAATCGAACTTGTCTATCAGGGAAACATGTCCCGTAGGAAACAGACAAATCCAAATAAAGTTCACT
Seq A exon
GGGATCAAGTATTTGCTGGGTTAGAAGAACAAGCCCGACAGGCAATGATGAAAACCGAGTTTCCTGGAGACCTTGGCAGTCAGCGACAAGCTCTCCAACATCTAAGAGACCAGGACTCCAGTAGCA
Seq C2 exon
GTGACAGTGAAGGTGACGAAGAGGAGACCACACAAGATGAAGTATCTTCCCACACATCAGAGGAAGATGGTGGGGTAGTCAAAGTGGAAAGAGAATTAGACAGTGCAGAAGCACCTGTCGGTAGTGGGGACGCACTGGTGGAGCACGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000262_MULTIEX1-1/4=C1-2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGGGGTGAAACTGGGAAAGA
R:
CTCGTGCTCCACCAGTGC
Band lengths:
234-360
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.846, FDR=0.038), PC9 No Change at 14 days (FC=0.953, FDR=0.584), Late Xenograft Enriched (FC=1.741, FDR=0.002).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]