MmuEX0053641 @ mm10
Exon Skipping
Gene
ENSMUSG00000031728 | Zfp821
Description
zinc finger protein 821 [Source:MGI Symbol;Acc:MGI:1923121]
Coordinates
chr8:109709411-109721343:+
Coord C1 exon
chr8:109709411-109709527
Coord A exon
chr8:109717762-109717887
Coord C2 exon
chr8:109721195-109721343
Length
126 bp
Sequences
Splice sites
3' ss Seq
CTTTTTCTTGTGATCACCAGGGG
3' ss Score
7.91
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
GTCAGCACCGTACGAGGAGGTAGTGACCTGTCCAGGGGTGAAACTGGGGAAGAACGGAGCTTGGATATCAGGGAAACATGTCCCGTAGGAAACAGACAAACCCAAATAAAGTTCACT
Seq A exon
GGGATCAAGTCTTTGCTGGGTTAGAAGAACAAGCCCGACAGGCAATGATGAAAACCGATTTTCCTGGAGACCTTGGCAGTCAGCGACAAGCTATCCAACAACTACGAGATCAGGACTCCAGTAGCA
Seq C2 exon
GTGACAGTGAAGGTGACGAAGAGGAGACCACACAAGATGAAGTATCTTCCCACACATCAGAGGAAGATGGTGGGGTAGTCAAAGTGGAAAAGGAATTAGAGACTGCGGAAGCACCTGTCAGTAGTGGGGACGCACTAGCAGAGCGCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031728_MULTIEX1-4/6=2-5
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.821 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
ENSMUST00000034163fB20022
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCGTACGAGGAGGTAGTGAC
R:
CTAGTGCGTCCCCACTACTGA
Band lengths:
249-375
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.846, FDR=0.038), PC9 No Change at 14 days (FC=0.953, FDR=0.584), Late Xenograft Enriched (FC=1.741, FDR=0.002).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types