GgaEX0000801 @ galGal4
Exon Skipping
Gene
ENSGALG00000000817 | ZNF821
Description
zinc finger protein 821 [Source:HGNC Symbol;Acc:HGNC:28043]
Coordinates
chr11:19175526-19182465:+
Coord C1 exon
chr11:19175526-19175611
Coord A exon
chr11:19181694-19181819
Coord C2 exon
chr11:19182323-19182465
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTTGTCCTTGTGGCTGCCAGGGG
3' ss Score
6.99
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
CCTCTGCTGAGGGAACGAACAGACTGTGCTCCCAGGTCAGGGAAACATGTCCCGTCGGAAACAGACCACTCCTAACAAAGTTCACT
Seq A exon
GGGAGCAAGTCTTTGCGGGGCTGGAGGAGCAAGCCCGCCAAGCCATGATGAAAAACAGCTTTCCTGGAGCTCTTGGGGACCAAAGGCCAGCTATTCATCCACTGCAAGACCGCGATTTCAGCAGCA
Seq C2 exon
GTGGCAGTGATGATGAGGAAACCACCCACGATGAAGTTTCTTCCCATACTTCAGAGGAGGATGGCTCAATGCTAAAGGTGAAGAAAGAACTGGAGAATGCAGAACAACATGCAGCTGGAACCCCACTGATGAGAGAGAATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000000817_MULTIEX1-3/3=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
ENSGALT00000001191fB3397
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGCTGAGGGAACGAACAGA
R:
TCATTCTCTCTCATCAGTGGGGT
Band lengths:
226-352
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.846, FDR=0.038), PC9 No Change at 14 days (FC=0.953, FDR=0.584), Late Xenograft Enriched (FC=1.741, FDR=0.002).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]