HsaEX0074408 @ hg38
Exon Skipping
Gene
ENSG00000102984 | ZNF821
Description
zinc finger protein 821 [Source:HGNC Symbol;Acc:HGNC:28043]
Coordinates
chr16:71864903-71880023:-
Coord C1 exon
chr16:71879907-71880023
Coord A exon
chr16:71867912-71868037
Coord C2 exon
chr16:71864903-71865048
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTTTTCCTTGTGACCACCAGGGG
3' ss Score
7.49
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
GTTAGCATCTTGCAAGGAGGTAGTAACATATCCAGTCGTGAAACTGGGAAAGACCAGAGCTCTTGCATCAGGGAAACATGTCCCGTCGGAAACAGACAAATCCAAATAAAGTTCACT
Seq A exon
GGGATCAAGTATTTGCTGGGCTAGAAGAGCAAGCCCGCCAGGCGATGATGAAAACTGATTTTCCTGGAGACCTTGGCAGTCAGCGACAAGCTATCCAACAACTAAGAGATCAGGACTCCAGTAGCA
Seq C2 exon
GTGACAGTGAGGGTGATGAAGAGGAGACGACACAAGATGAAGTCTCTTCCCACACATCAGAGGAAGATGGAGGGGTGGTCAAAGTGGAGAAAGAGTTAGAAAATACAGAACAGCCTGTTGGTGGGAACGAAGTGGTAGAGCACGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000102984-'10-27,'10-25,16-27=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.936 C2=0.992
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Inclusion Isoforms:
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATCTTGCAAGGAGGTAGTAACA
R:
CGTGCTCTACCACTTCGTTCC
Band lengths:
257-383
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.846, FDR=0.038), PC9 No Change at 14 days (FC=0.953, FDR=0.584), Late Xenograft Enriched (FC=1.741, FDR=0.002).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- The Cancer Genome Atlas (TCGA)
- Genotype-Tissue Expression Project (GTEx)
- Autistic and control brains
- Pre-implantation embryo development