MmuEX1006286 @ mm10
Exon Skipping
Gene
ENSMUSG00000032477 | Cdc25a
Description
cell division cycle 25A [Source:MGI Symbol;Acc:MGI:103198]
Coordinates
chr9:109879231-109881577:+
Coord C1 exon
chr9:109879231-109879267
Coord A exon
chr9:109879837-109879935
Coord C2 exon
chr9:109881473-109881577
Length
99 bp
Sequences
Splice sites
3' ss Seq
CTGTTTTACTTTGATAGCAGAAG
3' ss Score
2.55
5' ss Seq
AATGTAAGC
5' ss Score
6.12
Exon sequences
Seq C1 exon
CCTTGAAATTTCCCTGACGAGAATAAATTCCCTACCT
Seq A exon
AAGCTCCTGGGATGTAGCCCAGCCCTGAAGAGGAGCCACTCTGATTCTCTAGACCACGACACCTTTCACCTCATCGACCAGGATGAAAATAAAGAAAAT
Seq C2 exon
GAAGCATTTGAATTTAAAAAGCCAATACGACCTGCATCTCGTCACATCTACGAGGAGAGTAAAGATCCCTTTACACACAGGCAGAATTCAGCCCCAGCTCGGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032477-'4-7,'4-6,5-7
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.439 A=0.640 C2=0.829
Domain overlap (PFAM):
C1:
PF066178=M-inducer_phosp=FE(5.1=100)
A:
PF066178=M-inducer_phosp=FE(14.0=100)
C2:
PF066178=M-inducer_phosp=FE(14.5=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTTGAAATTTCCCTGACGAGA
R:
GCTGGGGCTGAATTCTGC
Band lengths:
135-234
Functional annotations
There are 1 annotated functions for this event
PMID: 12411508
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, detection by mass spectrometry, inhibitor, protein kinase assay. ELM ID: ELMI003208; ELM sequence: LGCSPALK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types